Cyanine5.5 NHS ester | 1469277-96-0

Catalog Number	R01-0021
Category	Cyanine5.5
Molecular Formula	C44H46N3ClO4
Molecular Weight	716.32

Catalog Number	Size	Price	Quantity
R01-0021	5 mg	$298
R01-0021	--	--

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Product Introduction

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester. Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

Chemical Information
Product Specification
Application

Related CAS	2054318-82-8 (E,Z,E-chloride) 1874250-60-8 (E,Z,E-without anion) 1469541-53-4 (without anion)
Purity	NMR 1H, HPLC-MS (95%)
Solubility	soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)
Appearance	dark blue solid

ε, L⋅mol-1⋅cm-1	198000
Fluorescence Quantum Yield	0.2
Excitation	684
Emission	710
Storage	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cyanine5.5 NHS ester is widely used in fluorescence imaging due to its exceptional brightness and stability. Its strong absorbance and emission in the near-infrared range allow for deep tissue penetration, making it an ideal choice for in vivo imaging applications. Researchers employ Cyanine5.5 to label biomolecules such as proteins, peptides, and nucleic acids, facilitating real-time tracking and visualization of cellular processes. This application is particularly valuable in cancer research, where understanding tumor dynamics and metastasis can lead to improved treatment strategies.

Another significant application of Cyanine5.5 NHS ester is in the development of biosensors. Its ability to form stable covalent bonds with biomolecules enhances the sensitivity and specificity of these sensors. By incorporating Cyanine5.5 into biosensor platforms, researchers can detect low-abundance targets in complex biological samples, such as proteins or small molecules. This capability is crucial in diagnostics and therapeutic monitoring, enabling the detection of diseases at earlier stages and improving patient outcomes through timely interventions.

Cyanine5.5 NHS ester also plays a critical role in the field of drug delivery. By conjugating this fluorescent dye to drug molecules or carriers, researchers can track the distribution and release profiles of therapeutics within biological systems. This tracking is essential for optimizing drug formulations and ensuring the effective delivery of medications to target sites. The fluorescent properties of Cyanine5.5 provide valuable insights into the pharmacokinetics and biodistribution of drugs, paving the way for more effective treatment regimens in various diseases, including cancer and autoimmune disorders.

Lastly, Cyanine5.5 NHS ester is increasingly utilized in multiplexing applications. In diagnostic assays, the ability to label multiple targets with different fluorescent dyes allows for simultaneous detection and quantification. Cyanine5.5’s compatibility with other fluorophores enables the development of complex assays, such as multiplexed PCR or ELISA, enhancing the throughput and efficiency of diagnostic workflows. This multiplexing capability is particularly advantageous in clinical settings, where comprehensive profiles of biomarkers can aid in personalized medicine approaches and better therapeutic decisions.

Applications of Fluorescent Probes & Dyes