C12 NBD dihydro Ceramide | 474943-05-0

Catalog Number	A18-0061
Category	Fluorescent Enzyme Substrates
Molecular Formula	C36H63N5O6
Molecular Weight	661.92

Catalog Number	Size	Price	Quantity
A18-0061	--	$--

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Product Introduction

C12 NBD dihydro ceramide is a fluorescent ceramide derivative, which can be used as a fluorogenic substrate for the measurement of alkaline and neutral ceramidase activity from a variety of sources. C12 NBD dihydro ceramide may exhibit reduced activity in ceramidase assays compared to C-12 NBD ceramide, for its saturated bond in the C4-C5 position of the sphingosine backbone.

Chemical Information
Product Specification
Application

Synonyms	C-12 NBD-dihydro-Ceramide; C12-NBD Sphinganine; N-[(1S,2R)-2-hydroxy-1-(hydroxymethyl)heptadecyl]-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-dodecanamide; 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
Purity	≥98%
Shelf Life	1 Year
IUPAC Name	N-[(2S,3R)-1,3-dihydroxyoctadecan-2-yl]-12-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]dodecanamide
Canonical SMILES	CCCCCCCCCCCCCCCC(C(CO)NC(=O)CCCCCCCCCCCNC1=CC=C(C2=NON=C12)[N+](=O)[O-])O
InChI	InChI=1S/C36H63N5O6/c1-2-3-4-5-6-7-8-9-10-12-15-18-21-24-33(43)31(29-42)38-34(44)25-22-19-16-13-11-14-17-20-23-28-37-30-26-27-32(41(45)46)36-35(30)39-47-40-36/h26-27,31,33,37,42-43H,2-25,28-29H2,1H3,(H,38,44)/t31-,33+/m0/s1
InChI Key	STPDREBSVHTICB-CQTOTRCISA-N
Appearance	Powder

Storage

Store at -20°C

C12 NBD dihydro Ceramide is a fluorescent lipid analog that is prominently used in diverse scientific studies. Here are some key applications of C12 NBD dihydro Ceramide:

Cellular Trafficking Studies: C12 NBD dihydro Ceramide is often used to study lipid transport and distribution within cells. Its fluorescence properties allow researchers to visualize and track the movement of ceramides and other sphingolipids across cellular membranes. This provides insights into cellular lipid dynamics and their roles in cell signaling and homeostasis.

Metabolic Research: This analog is instrumental in studies focusing on sphingolipid metabolism and its regulatory mechanisms. By incorporating C12 NBD dihydro Ceramide into cellular systems, researchers can observe its conversion into other sphingolipid species and measure enzyme activities. Understanding these metabolic pathways is crucial for elucidating the roles of sphingolipids in various diseases, such as cancer and metabolic disorders.

Apoptosis Studies: Scientists employ C12 NBD dihydro Ceramide to investigate ceramide’s role in cell apoptosis and programmed cell death. Its ability to mimic natural ceramides while being easily traceable due to its fluorescence makes it ideal for examining the pathways through which ceramides influence apoptosis. This information is vital for cancer research and the development of therapeutics targeting apoptotic pathways.

Membrane Dynamics: C12 NBD dihydro Ceramide is used to study membrane properties and dynamics due to its integration into lipid bilayers. Researchers can assess how ceramides affect membrane fluidity, structure, and interactions with proteins. This contributes to a better understanding of membrane-associated processes and their implications in health and disease.

Applications of Fluorescent Probes & Dyes