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AF488 NHS ester
| Catalog Number | R01-0001 |
| Category | Alexa Fluor |
| Molecular Formula | C31H32N4O13S2 |
| Molecular Weight | 732.74 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy. From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5-and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though-otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.
Chemical Information
Product Specification
Computed Properties
| Synonyms | 3,6-Diamino-9-(2-carboxy-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)phenyl)-5-sulfoxanthylium-4-sulfonate |
| Purity | NMR 1H, HPLC-MS (80+%, balance mostly carboxylic acid) |
| IUPAC Name | 3-amino-6-azaniumylidene-9-[2-carboxy-4-(2,5-dioxopyrrolidin-1-yl)oxycarbonylphenyl]-5-sulfoxanthene-4-sulfonate |
| Canonical SMILES | C1CC(=O)N(C1=O)OC(=O)C2=CC(=C(C=C2)C3=C4C=CC(=[NH2+])C(=C4OC5=C3C=CC(=C5S(=O)(=O)[O-])N)S(=O)(=O)O)C(=O)O |
| InChI | InChI=1S/C25H17N3O13S2/c26-15-5-3-12-19(11-2-1-10(9-14(11)24(31)32)25(33)41-28-17(29)7-8-18(28)30)13-4-6-16(27)23(43(37,38)39)21(13)40-20(12)22(15)42(34,35)36/h1-6,9,26H,7-8,27H2,(H,31,32)(H,34,35,36)(H,37,38,39) |
| InChIKey | FYCGGEVYPRTFDS-UHFFFAOYSA-N |
| Solubility | good in water, DMF, DMSO |
| Appearance | dark orange solid |
| ε, L⋅mol-1⋅cm-1 | 71800 |
| Fluorescence Quantum Yield | 0.91 |
| Excitation | 495 |
| Emission | 519 |
| XLogP3 | -0.6 |
| Hydrogen Bond Donor Count | 4 |
| Hydrogen Bond Acceptor Count | 14 |
| Rotatable Bond Count | 6 |
| Exact Mass | 631.02027995 g/mol |
| Monoisotopic Mass | 631.02027995 g/mol |
| Topological Polar Surface Area | 290Ų |
| Heavy Atom Count | 43 |
| Formal Charge | 0 |
| Complexity | 1590 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
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