6-Carboxyfluorescein diacetate | CAS 3348-03-6

6-Carboxyfluorescein diacetate (CFDA) is a versatile fluorescent dye widely utilized in diverse biological and biochemical settings. Here we explore four key applications of CFDA:

Cell Viability Assays: CFDA serves as a cornerstone for evaluating cell viability and cytotoxicity across various cell types. Upon cellular uptake, CFDA undergoes hydrolysis by intracellular esterases, unveiling the fluorescent derivative 6-carboxyfluorescein. This fluorescence can be quantified, offering researchers a window into the realm of viable cells within a population.

Flow Cytometry: Delving into the realms of cell tracking and proliferation studies, 6-Carboxyfluorescein diacetate finds extensive application in flow cytometry. Following its conversion to a fluorescent entity within cells, it becomes a beacon for traversing through multiple cell divisions. By tracking the dilution of fluorescence intensity, scientists can gain insights into cell proliferation rates and lineage tracing dynamics.

Intracellular pH Measurement: A realm where CFDA truly shines is in the measurement of intracellular pH levels. Its fluorescence intensity is intricately linked with pH variations, offering researchers a tool for real-time monitoring within cells. By introducing CFDA into cellular landscapes, it undergoes hydrolysis to release the pH-sensitive dye 6-carboxyfluorescein, unveiling a dynamic palette for exploring intracellular pH fluctuations across diverse experimental terrains.

Confocal Microscopy: Entering the realm of confocal microscopy, CFDA emerges as a vital ally for imaging live cells and tissues with finesse. Its exceptional fluorescence properties, coupled with non-toxicity and cell membrane penetration capabilities, make it a prized asset for live-cell imaging endeavors. By harnessing CFDA, researchers can peer into the intricate world of cellular processes such as endocytosis, organelle dynamics, and cellular motility, painting a vivid picture with high spatial resolution.