3,3'-Dihexyloxacarbocyanine iodide | 53213-82-4

3,3’-Dihexyloxacarbocyanine iodide (commonly known as DiOC6) is a fluorescent dye used extensively in bioscience research. Here are some key applications of 3,3’-Dihexyloxacarbocyanine iodide:

Mitochondrial Membrane Potential Measurement: DiOC6 is frequently used to assess mitochondrial membrane potential in live cells. By accumulating in mitochondria, the dye allows researchers to visualize and quantify changes in membrane potential. This application is vital for studying mitochondrial function and diagnosing mitochondrial diseases.

Cell Membrane Staining: DiOC6 is suitable for staining cell membranes, making it a valuable tool for fluorescence microscopy and flow cytometry. The dye aids in identifying and distinguishing different cell populations based on membrane characteristics. This application is particularly useful in immunology and cancer research, where cell membrane properties are key biomarkers.

Live Cell Imaging: DiOC6 can be used in live cell imaging to monitor intracellular structures and processes in real-time. Its compatibility with various imaging techniques makes it a versatile dye for studying cellular dynamics. Researchers can track cell morphology, migration, and interactions, providing insights into cellular behavior under various conditions.

Apoptosis Detection: DiOC6 dye can be employed to detect apoptosis by measuring disruptions in mitochondrial membrane potential, an early event in programmed cell death. This application is crucial for understanding the mechanisms of apoptosis and evaluating the efficacy of anti-cancer drugs. By detecting early apoptosis, researchers can also study cell survival and death pathways, contributing to targeted therapy development.