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Rhod-5N potassium salt
| Catalog Number | A14-0024 |
| Category | Calcium, Chloride and Other indicators |
| Molecular Formula | C39H36K3N5O13 |
| Molecular Weight | 900.0 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0024 | -- | -- |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Rhod-5N is a low affinity fluorescent calcium probe (Kd = 320 µM), which is commonly used for studying compartments with high concentrations of calcium.
Chemical Information
Product Specification
Application
| Synonyms | 2,2'-((2-(2-(2-(bis(carboxylatomethyl)amino)-5-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)phenoxy)ethoxy)-4-nitrophenyl)azanediyl)diacetate |
| Purity | ≥90% |
| Canonical SMILES | CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](CC(=O)[O-])CC(=O)[O-])C(=C3)OCCOC4=C(C=CC(=C4)[N+](=O)[O-])N(CC(=O)[O-])CC(=O)[O-])C5=C(O2)C=C(C=C5)N(C)C.[K+].[K+].[K+] |
| InChI | InChI=1S/C39H39N5O13.3K/c1-40(2)24-6-9-27-31(16-24)57-32-17-25(41(3)4)7-10-28(32)39(27)23-5-11-29(42(19-35(45)46)20-36(47)48)33(15-23)55-13-14-56-34-18-26(44(53)54)8-12-30(34)43(21-37(49)50)22-38(51)52;;;/h5-12,15-18H,13-14,19-22H2,1-4H3,(H3-,45,46,47,48,49,50,51,52);;;/q;3*+1/p-3 |
| InChIKey | JLZGTFWLMRDWKD-UHFFFAOYSA-K |
| Appearance | Solid Powder |
| Excitation | 551 nm |
| Emission | 576 nm |
| Storage | Store at -20°C |
Rhod-5N potassium salt, a fluorescent dye utilized in various bioscience applications, is instrumental in measuring intracellular potassium levels. Here are four key applications of Rhod-5N potassium salt:
Live-Cell Imaging: Employing Rhod-5N potassium salt for visualizing and quantifying potassium ion fluxes in live cells allows researchers to track real-time changes in potassium levels using fluorescence microscopy. This method is essential for investigating intricate cellular processes like neuron firing, cardiac muscle contraction, and hormone secretion.
Electrophysiological Studies: In the realm of electrophysiology, Rhod-5N potassium salt plays a crucial role in evaluating the dynamics of potassium ions in cells under diverse conditions. By using this dye, researchers can correlate ion fluxes with the electrical activity in excitable tissues such as neurons and cardiac cells, shedding light on the mechanisms of electrical signaling and the impacts of drugs on ion channels.
Drug Screening: Leveraging Rhod-5N potassium salt in high-throughput drug screening assays enables the assessment of compounds’ effects on potassium ion transport and channel activity. Through monitoring changes in fluorescence, researchers can pinpoint potential drug candidates that modulate ion channels, particularly crucial for developing treatments for ailments like epilepsy, arrhythmias, and hypertension.
Cell Viability Assessment: Rhod-5N potassium salt serves as a valuable tool for evaluating cell viability by quantifying intracellular potassium levels, which fluctuate during cellular processes like apoptosis or necrosis. Reduced fluorescence signals can indicate compromised cellular integrity and functionality, offering a non-invasive method to assess compound cytotoxicity in research and development settings.
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