Pacific Blue Dyes
Pacific Blue, or systemically called 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its maximum excitation wavelength is 401 nm and its maximum emission wavelength is 452 nm. Even at a pH of about 7, its fluorescence is still very high. Pacific Blue is a member of the Pacific Dye Group. Pacific dyes include Pacific Orange, Pacific Green, and Pacific Blue. The maximum absorption of these fluorescent dyes is between 400 and 410 nm, but they have different emission spectra: this allows simultaneous excitation with one laser, which produces maximum emission of 455 nm, 500 nm, and 551 nm, respectively (Note: maximum absorption emission May vary by manufacturer. In flow cytometry, Pacific Blue (and other Pacific dyes) are typically absorbed at 405 nm, and are not allowed with other fluorophores (such as BV421 or V450) that share similar excitation and / or emission spectra use simultaneously.
Figure 1. Chemical structure of Pacific Blue dyes.
Applications
In general, Pacific Blue is used as a reactive form for immunohistochemistry or targeted staining and is capable of binding to targeted antibodies. The reaction form used is Pacific Blue succinimide ester or Pacific Blue C5-maleimide.
BOC Sciences provides Pacific Blue dyes that can be conjugated to a variety of antibodies, peptides, proteins, tracers and amplification substrates to optimize cell labeling and detection. In addition, reactive dye and protein labeling kits are provided so you can produce your own antibody conjugates or probes.
Reference:
- Lee, Molly M.; et al. Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore. ACS Omega. 2016, 1: 1266–1276.
Ion Probe
- Calcium Probe
- Chloride Probes
- Copper Probes
- Fluoride Probe
- Iron (III) Probes
- Magnesium Probes
- Sodium Probes
- Zinc Probes
Metal Probe
Organelle Probe
- Endoplasmic Reticulum Fluorescent Probe
- Golgi Fluorescent Probe
- Lysosomal Fluorescent Probe
- Mitochondrial Fluorescent Probe
Other Probes
- Cyanide Probes
- Cysteine Probe
- Fluo-2 AM Probes
- Fluo-2 Probes
- Fluo-3 AM Probes
- Fluo-3 Probes
- Fluorescent Probes for Imaging Bacteria
- Fluorescent Probes for Imaging Bacteria
- Glucose Probes
- Glucose Probes
- Glutathione (GSH) Probes
- Glutathione (GSH) Probes
- Indo-1 Probes
- Nitric Oxide Probes
- Nitric Oxide Probes
- Viscosity Probes
- Viscosity Probes
PH Probe