Iron (III) Probes

BOC Sciences offers a range of highly photostable probes (fluorescent dye molecules) sensitive to Iron (Fe³⁺) ions. Our probes are very bright (highly fluorescent), easy to use and provide highly reproducible results. Our probes can be used in an Absorption (colorimetric) or Fluorescence manner. Our probes do not react with iron like other approaches for Iron detection, but more simply, Fe³⁺ readily binds, causing dramatic changes / shifts in the absorption spectrum or in fluorescence intensity. Our probes are offered in our standard vial format, ideal for nearly all analysis formats such as in a cuvette or in microscopy; in a 96-well kit, and pre-loaded into a box of 100 cuvettes for quick, easy and convenient Iron analysis. A reconstitution and use data sheet is provided with each probe.

BOC Sciences offers a unique fluorescence probe, that is selective for Iron (Fe³⁺) over other dissolved cations, that can both detect and quantitate dissolved Iron (Fe³⁺ ions). Iron (III) Probes is not influenced by Fe2+ ions, enabling the two forms of Iron to be readily distinguished by this remarkable fluorescent probe. Iron (III) Probes is a turn-on fluorescent sensor, meaning that the solution fluorescence increases as a function of the concentration of dissolved Iron and as a function of time. Iron (III) Probes can readily be excited over a broad wavelength range from 280-400 nm with an extinction coefficient of 23 000 ± 3 000M^-1 cm^-1 at 386 nm. The fluorescence emission is very strong in the presence of Fe³⁺ ions, centered at about 430 nm. It can be used to readily quantitate the amount of Fe³⁺ in a sample by using a given time point to record the emission intensity or alternatively by using the equilibrium fluorescence intensities to record the calibration curve. For concentration greater than 50 µM (10 equivalents of Iron to dye), the equilibrium is achieved in less than 8 minutes. It is a selective probe for both the detection and determination of Fe³⁺ ions. We offer it already conveniently loaded into a box of 100 plastic cuvettes (transmission down to 285 nm). These cuvettes allow for dissolved Fe³⁺ detection in either an absorption or fluorescence based format. With these convenient pre-loaded cuvettes, there is no reconstitution procedure necessary, simply add 3 ml (3cm3) of your sample to each cuvette and measure, it's that simple. We offer it in a convenient 96-well kit format. A clear bottom 96-well plate and a vial are supplied, allowing up to 96 Fe³⁺ measurements to be undertaken in an absorption- based manner. Iron (III) Probes in a 96-well Format (For Fluorescence). We offer Iron (III) Probes in a convenient 96-well kit format. A black 96-well plate and a vial are supplied, allowing up to 96 Fe³⁺ measurements to be undertaken in a fluorescence-based manner.

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