Sulfo-Cyanine7.5 dicarboxylic acid

Sulfo-Cyanine7.5 dicarboxylic acid is a water-soluble, sulfonated cyanine dye designed for fluorescent labeling and imaging workflows where strong near-infrared signal is advantageous. Its dicarboxylic acid functionality supports conjugation strategies to biomolecules and targeting constructs, enabling robust fluorescent tracking in microscopy, imaging, and fluorescence-based assays. As a sulfonated cyanine, it is frequently selected for applications requiring aqueous compatibility and reduced aggregation compared with less polar cyanines.

1. Fluorescence Imaging Tracers

Sulfo-Cyanine7.5 dicarboxylic acid is widely used as a near-infrared fluorescent tracer for labeling biomolecules, polymers, and nanoparticles to visualize distribution and transport in optical imaging experiments. Researchers in chemical biology and biomaterials commonly incorporate the dye into conjugates for tracking uptake, localization, and clearance in in vitro imaging formats, leveraging the dye's NIR emission for reduced background relative to visible fluorophores. The sulfonated, dicarboxylic acid structure also supports aqueous handling during probe preparation and downstream assay workflows.

2. Bioconjugation Labeling Reagents

Sulfo-Cyanine7.5 dicarboxylic acid serves as a fluorescent tag for constructing dye-labeled proteins, peptides, and other research reagents used in binding studies and fluorescence readouts. In typical bioconjugation workflows, the dicarboxylic acid functionality is leveraged to generate fluorescent conjugates that can be used in labeling panels for assay development, reagent characterization, and molecular imaging reagent screening. Because the dye is designed for aqueous compatibility, it is frequently selected when conjugation and labeling must be performed in buffered conditions compatible with sensitive biomolecules.

3. Fluorescence-Based Bioassays

Sulfo-Cyanine7.5 dicarboxylic acid is commonly employed as a reporter dye in fluorescence assays where near-infrared emission is used to monitor binding, transport, or labeling-dependent signal changes. Assay developers use dye-labeled biomolecular reagents to quantify interactions in plate-based formats, to follow reagent incorporation into complex mixtures, and to support imaging readouts that benefit from NIR spectral separation. The dye's ionic character and NIR properties make it a practical choice for fluorescence measurements where visible-channel interference is a concern.

4. Nanoparticle And Polymer Labeling

Sulfo-Cyanine7.5 dicarboxylic acid is used to fluorescently label nanomaterials and polymeric systems for materials characterization and molecular imaging reagent development. Researchers in biomaterials science incorporate the dye into labeled coatings, conjugated polymers, or surface-associated nanoparticle constructs to study dispersion, stability, and functionalization outcomes using fluorescence microscopy or optical readouts. The dye's sulfonated, dicarboxylic acid features help support incorporation into aqueous processing workflows and facilitate consistent labeling for comparative materials studies.