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Phalloidin-ATTO 594
| Catalog Number | F10-0150 |
| Category | ATTO Dyes |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| F10-0150 | -- | -- |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
ATTO 594 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, excellent water solubility, and very little triplet formation. After coupling to a substrate ATTO 594 carries a net electrical charge of -1.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.find more information here
Chemical Information
Product Specification
Application
| Purity | ≥90% (HPCE) |
| NACRES | NA.32 |
| Excitation | 601 |
| Emission | 627 |
| Properties Quality Level | 100 |
| Storage | −20 °C |
Phalloidin-ATTO 594, a toxin labeled with fluorescence, exhibits specificity in binding to filamentous actin (F-actin) within cells, establishing itself as a potent asset in fluorescence microscopy. Here are four key applications of Phalloidin-ATTO 594:
Cell Structure Visualization: Utilizing Phalloidin-ATTO 594, researchers stain and scrutinize the structure and arrangement of the cytoskeleton across diverse cell types. By targeting F-actin with precision, this tool allows for the vivid visualization of actin filaments marked by pronounced contrast under a fluorescence microscope. This aids in the exploration of cell morphology, dynamics, and interactions within the intricate framework of cellular architecture.
Actin Dynamics Research: Within the realm of cell motility and migration studies, Phalloidin-ATTO 594 emerges as a key player, facilitating the investigation of actin filament dynamics. Through time-lapse fluorescence microscopy, researchers can track the fluctuations in the actin cytoskeleton over time, offering invaluable insights into processes like cell division, polarization, and response to external cues. This insight is pivotal for unraveling the underlying mechanisms governing cell movement and behavior.
Comparative Cytoskeletal Studies: Scientists leverage Phalloidin-ATTO 594 to delve into the comparative analysis of cytoskeletal organization across different cell types or under diverse experimental settings. By quantifying the distribution and alignment of actin filaments, researchers can evaluate the impacts of genetic alterations, pharmaceutical interventions, or environmental influences on the cytoskeleton. This application serves as a cornerstone for drug screening initiatives and mechanistic investigations in cell biology.
Tissue Imaging: Beyond cell cultures, Phalloidin-ATTO 594 extends its reach to the realm of tissue staining for histological examinations. It offers a window into the visualization of actin structures within the context of tissue architecture, furnishing a comprehensive blueprint of cellular layout within organs. This facet proves invaluable for endeavors in developmental biology, tissue engineering, and pathology, enriching our understanding of tissue organization and cellular dynamics.
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