BHQ-1 amine | CAS 1308657-79-5

BHQ-1 amine is a fluorescence quencher building block designed for constructing quencher-labeled oligonucleotides, peptides, and other bioconjugates where signal suppression is required. As an amine-functionalized BHQ quencher, it is commonly used to position a quencher in close proximity to a fluorophore to enable distance-dependent fluorescence control in fluorescence-based assays and molecular imaging workflows.

1. Molecular Beacon Probes

BHQ-1 amine is frequently incorporated into molecular beacon designs used to monitor nucleic acid hybridization by fluorescence recovery upon target binding. Researchers attach BHQ-1 amine to the oligonucleotide scaffold so that the quencher resides near the reporter dye in the closed state, reducing background fluorescence and improving interpretability of hybridization-dependent signal changes during fluorescence readouts.

2. Oligonucleotide FRET Assays

BHQ-1 amine is also used in FRET-based assay formats where a quencher-labeled oligonucleotide or oligonucleotide conjugate controls fluorescence output from a nearby reporter dye. In these workflows, the amine handle supports straightforward conjugation to biomolecular carriers, enabling quencher placement for controlled energy transfer and signal modulation in nucleic-acid analysis, probe optimization, and assay development.

3. Real-Time Nucleic Acid Probes

BHQ-1 amine is applied as a quencher component in real-time fluorescence probe architectures that rely on quenched baseline emission and target-dependent fluorescence changes. Molecular biology groups use BHQ-1 amine-labeled oligos to construct probe sets for fluorescence-based nucleic acid workflows, including assay reagent development where minimizing off-target or unbound signal is important for robust data interpretation.

4. Fluorescent Reporter Suppression

BHQ-1 amine is used more broadly as a quencher conjugation reagent for suppressing fluorescence in labeled biomolecule constructs beyond nucleic acids, including quencher-fluorophore reporter pairs used in chemical biology experiments. The amine functionality enables coupling to amine-reactive labeling strategies on peptides, linkers, and other bioconjugation scaffolds to generate defined quencher-reporter proximity for controlled fluorescence output in assay and imaging reagent development.