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Ac-ANW-AMC
| Catalog Number | A18-0049 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C30H32N6O7 |
| Molecular Weight | 588.61 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Ac-ANW-AMC is a 7-amino-4-methylcoumarin labelled fluorogenic peptidyl substrate and used for measuring chymotrypsin-like activity of the immunoproteasome.
Chemical Information
Product Specification
Application
Computed Properties
| Synonyms | (S)-N1-((S)-3-(1H-indol-3-yl)-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxopropan-2-yl)-2-((S)-2-acetamidopropanamido)succinamide; N-Acetyl-L-alanyl-L-asparaginyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)-L-tryptophanamide; Acetyl-Ala-Asn-Trp-7-amido-4-methylcoumarin |
| Purity | >99% |
| IUPAC Name | (2S)-2-[[(2S)-2-acetamidopropanoyl]amino]-N-[(2S)-3-(1H-indol-3-yl)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopropan-2-yl]butanediamide |
| Canonical SMILES | CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CC3=CNC4=CC=CC=C43)NC(=O)C(CC(=O)N)NC(=O)C(C)NC(=O)C |
| InChI | InChI=1S/C30H32N6O7/c1-15-10-27(39)43-25-12-19(8-9-20(15)25)34-29(41)23(11-18-14-32-22-7-5-4-6-21(18)22)36-30(42)24(13-26(31)38)35-28(40)16(2)33-17(3)37/h4-10,12,14,16,23-24,32H,11,13H2,1-3H3,(H2,31,38)(H,33,37)(H,34,41)(H,35,40)(H,36,42)/t16-,23-,24-/m0/s1 |
| InChIKey | ORTKHFLJSJQDKO-ZCWWJEROSA-N |
| Solubility | Soluble in DMSO |
| Density | 1.4±0.1 g/cm3 |
| Appearance | Lyophilized Powder |
| Boiling Point | 1119.0±65.0°C at 760 mmHg |
| Excitation | 340-360 nm |
| Emission | 440-460 nm |
| Storage | Store at -20°C |
Ac-ANW-AMC, a synthetic substrate employed in enzymatic assays to explore protease activity, finds diverse applications in research and clinical diagnostics.
Protease Activity Assays: Widely utilized in fluorometric assays, Ac-ANW-AMC serves as a pivotal tool for detecting and quantifying protease activity. Upon cleavage by a protease, it liberates a fluorescent product, facilitating the precise measurement of enzyme kinetics and activity levels. This capability is critical for the screening of protease inhibitors and investigating the intricate roles of proteases in a myriad of physiological and pathological processes.
Biotechnology Research: In the realm of biotechnology, Ac-ANW-AMC is instrumental for scrutinizing enzyme specificity and substrate preferences in vitro. By delivering a consistent and quantifiable response, it aids researchers in fine-tuning conditions for protease reactions and evaluating enzyme modifications or mutations. This contributes to the advancement of engineered enzymes with heightened or modified activity, tailored for industrial applications.
Drug Discovery: Acting as a cornerstone in drug development targeting proteases implicated in various diseases, Ac-ANW-AMC plays a crucial role. Through its application in high-throughput screening, researchers can pinpoint potential protease inhibitors that hold promise as therapeutics for ailments ranging from cancer to infectious diseases and inflammatory disorders. It facilitates a deeper comprehension of the efficacy and selectivity of candidate compounds, propelling advancements in pharmaceutical research.
Clinical Diagnostics: In clinical scenarios, Ac-ANW-AMC-based assays are indispensable for evaluating proteolytic activity in biological samples, aiding in diagnosing diseases characterized by altered protease expression or activity.
| XLogP3 | 0.4 |
| Hydrogen Bond Donor Count | 6 |
| Hydrogen Bond Acceptor Count | 7 |
| Rotatable Bond Count | 11 |
| Exact Mass | 588.23324738 g/mol |
| Monoisotopic Mass | 588.23324738 g/mol |
| Topological Polar Surface Area | 202Ų |
| Heavy Atom Count | 43 |
| Formal Charge | 0 |
| Complexity | 1140 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 3 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
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