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Pepstatin A, BODIPY FL Conjugate

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Pepstatin A, BODIPY FL Conjugate

Catalog Number	F01-0217
Category	BODIPY
Molecular Formula	C53H88BF2N9O9
Molecular Weight	1044.14

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* Please be kindly noted products are not for therapeutic use. We do not sell to patients.

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Product Introduction

Pepstatin A, BODIPY FL conjugate is a green-fluorescent probe for investigation of cathepsin D tracking in cells. Pepstatin A selectively binds to cathepsin D at pH 4.5.

Chemical Information
Product Specification
Application

Synonyms	Pepstatin A, EverFluor FL Conjugate
Appearance	Solid Powder

Excitation	553
Emission	575

Pepstatin A BODIPY FL Conjugate, a fluorescently labeled inhibitor, is a versatile tool for biochemical and cellular studies. Here are four key applications of this conjugate:

Protease Activity Assays: Widely utilized in the investigation of protease activity, particularly focusing on aspartic proteases like cathepsins, this conjugate binds to these enzymes, enabling researchers to visualize and quantify protease activity in real-time. Through fluorescence microscopy or flow cytometry, scientists can gain insights into protease function in both physiological and pathological contexts, shedding light on the intricate mechanisms at play.

Live Cell Imaging: With the BODIPY FL fluorophore, the Pepstatin A conjugate finds its application in live cell imaging, allowing for the tracking of protease activity within intact cells. Scientists can observe dynamic changes in protease behavior in response to stimuli or specific cellular events. This valuable tool aids in drug discovery and enhances our understanding of disease progression at a cellular level, offering a window into the inner workings of cellular processes.

Enzyme Localization Studies: Leveraged to pinpoint the subcellular localization of proteases within cells, the Pepstatin A BODIPY FL Conjugate’s fluorescence assists in accurately mapping enzyme distribution in organelles. This precise localization helps unravel the functional roles of proteases in various cellular compartments, contributing crucial insights to the understanding of complex cellular processes and the design of targeted therapies.

High-Throughput Screening: In high-throughput screening assays aimed at identifying potential inhibitors of aspartic proteases, this conjugate plays a pivotal role. By offering a fluorescent readout, it streamlines the evaluation of compound effects on protease activity, facilitating rapid screening of numerous compounds. Such screening plays a critical role in the discovery of new drug candidates for diseases characterized by dysregulated protease activity.