Lucifer Yellow CH dilithium salt | CAS 67769-47-5

Catalog Number	A16-0065
Category	Other Cell Fluorescent Probes
Molecular Formula	C13H9Li2N5O9S2
Molecular Weight	457.2

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Product Introduction

Lucifer yellow CH is a fluorescent probe that stains nerve cells. It is the hydrazine form of Lucifer Yellow VS and contains a free hydrazido group that reacts with aliphatic aldehydes. It has been used as a biological tracer for monitoring the branching pattern and regeneration of neurons, gap junction detection and characterization, and selective ablation of cells following aldehyde fixation.

Chemical Information
Product Specification
Application

Synonyms	Lucifer Yellow carbohydrazide; 6-amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid dilithium salt
Purity	≥80%
Canonical SMILES	[Li+].[Li+].C1=C(C=C2C3=C1C(=O)N(C(=O)C3=CC(=C2N)S(=O)(=O)[O-])NC(=O)NN)S(=O)(=O)[O-]
InChI	InChI=1S/C13H11N5O9S2.2Li/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2
InChIKey	RPKCZJYDUKVMGF-UHFFFAOYSA-L
Appearance	Solid Powder

Excitation	430 nm
Emission	540 nm
Storage	Store at -20°C

Lucifer Yellow CH dilithium salt, a fluorescent dye renowned for its exceptional labeling and tracing capabilities, finds widespread use in biological research. Here are four key applications of Lucifer Yellow CH dilithium salt:

Neuronal Tracing: A cornerstone in neuroscience, Lucifer Yellow CH serves as a potent tool for tracing neuronal pathways and unraveling the intricate web of nervous system connectivity. By injecting the dye into neurons, it permeates the cell, enabling scientists to visualize the labyrinthine neuronal structures and connections. This visualization is pivotal in deciphering the complexities of neuronal circuits and shedding light on the inner workings of the brain.

Cellular Microinjection: Intriguingly, Lucifer Yellow CH can be precisely microinjected into individual cells to delve into the mysteries of intracellular processes. The dye’s fluorescent nature simplifies tracking the distribution and movements of cellular components, empowering researchers to explore cell morphologies, intracellular transport dynamics, and intercellular communications with finesse.

Gap Junction Studies: Delving into cellular communication mechanisms, Lucifer Yellow CH features prominently in elucidating the function and permeability of gap junctions that bridge cells. When introduced into a solitary cell, this dye can traverse through functional gap junctions to neighboring cells, unveiling insights into cell-to-cell communication and unraveling the regulatory intricacies of gap junction channels with precision.

Live Cell Imaging: Positioned at the vanguard of live cell imaging, Lucifer Yellow CH dilithium salt emerges as a stalwart ally for real-time visualization of dynamic cellular processes. Owing to its intense fluorescence and stability, researchers can label live cells with this dye and capture unfolding cellular behaviors in exquisite detail under a fluorescence microscope. This real-time monitoring facilitates the study of cellular responses to stimuli, migration patterns, and division dynamics, enriching our understanding of cellular dynamics.