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Bz-FVR-AMC
| Catalog Number | A18-0026 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C37H43N7O6 |
| Molecular Weight | 681.8 |
| Catalog Number | Size | Price | Quantity |
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Product Introduction
Bz-FVR-AMC is a fluorogenic substrate for bovine cathepsins S and H, Xenopus endopeptidase XSCEP1, and esterase E-II from B. gabonica venom, among others. 7-amino-4-methylcoumarin (AMC) is released by cleavage of Bz-FVR-AMC and emits fluorescence that can be used to quantify enzyme activity.
Chemical Information |
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| Synonyms | Benzoyl-Phe-Val-Arg-AMC; Thrombin Fluorogenic Substrate III; N-((S)-1-(((S)-1-(((S)-5-guanidino-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxopentan-2-yl)amino)-3-methyl-1-oxobutan-2-yl)amino)-1-oxo-3-phenylpropan-2-yl)benzamide |
| Purity | ≥95% |
| Canonical SMILES | CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCCN=C(N)N)NC(=O)C(C(C)C)NC(=O)C(CC3=CC=CC=C3)NC(=O)C4=CC=CC=C4 |
| InChI | InChI=1S/C37H43N7O6/c1-22(2)32(44-35(48)29(20-24-11-6-4-7-12-24)43-33(46)25-13-8-5-9-14-25)36(49)42-28(15-10-18-40-37(38)39)34(47)41-26-16-17-27-23(3)19-31(45)50-30(27)21-26/h4-9,11-14,16-17,19,21-22,28-29,32H,10,15,18,20H2,1-3H3,(H,41,47)(H,42,49)(H,43,46)(H,44,48)(H4,38,39,40)/t28-,29-,32-/m0/s1 |
| InChI Key | QBDRNGBZKAOZHF-OLWNVYNHSA-N |
- Product Specification
- Application
| Storage | Store at -20°C |
Bz-FVR-AMC, a synthetic substrate commonly utilized in protease assays, to investigate enzyme activity. Here are four key applications of Bz-FVR-AMC:
Enzyme Activity Assays: Embraced in laboratories worldwide, Bz-FVR-AMC plays a pivotal role in evaluating the activity of proteases, particularly in research dedicated to unraveling the mysteries of protein degradation and processing. The enzymatic cleavage of Bz-FVR-AMC liberates a fluorescent compound, enabling quantitative measurement. This method empowers researchers to discern the kinetic properties and activity levels of specific proteases under diverse conditions, shedding light on the intricacies of enzyme functionality.
Drug Discovery and Development: In the realm of pharmaceutical exploration, Bz-FVR-AMC emerges as a critical tool for screening potential protease inhibitors, crucial for combating diseases like cancer and Alzheimer’s. By integrating Bz-FVR-AMC into high-throughput screening assays, scientists swiftly pinpoint compounds that effectively hinder target proteases. This expedites the drug discovery process and aids in the evaluation of therapeutic candidates, propelling advancements in the field of medicine.
Biochemical Research: Unveiling protease specificity and substrate preferences, Bz-FVR-AMC stands at the forefront of biochemical studies essential for comprehending enzyme function and regulation. Researchers navigate the intricate world of substrate binding sites and catalytic mechanisms of proteases using Bz-FVR-AMC and similar substrates. This invaluable information serves as the cornerstone for crafting specific inhibitors and fostering innovations within protease engineering, enriching our understanding of enzyme dynamics.
Clinical Diagnostics: Within the realm of clinical laboratories, Bz-FVR-AMC-based assays serve as a beacon for diagnosing disorders linked to aberrant protease activity, such as specific cancers and neurodegenerative ailments. This substrate facilitates sensitive and specific detection of protease activity in biological samples, offering profound diagnostic insights. These assays play a pivotal role in enhancing disease monitoring and patient care by uncovering protease activity levels tied to distinctive pathological conditions, paving the way for improved healthcare practices.
Applications of Fluorescent Probes & Dyes
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