![{[Name]}](https://resource.bocsci.com/structure/148185-53-9.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/137829-80-2.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/949108-72-9.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/105237-27-2.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/121461-69-6.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/vancomycin-bodipy-fl-conjugate.gif?v=2025011501)
Inquiry Basket
BODIPY TMR C5-maleimide
| Catalog Number | F01-0173 |
| Category | BODIPY |
| Molecular Formula | C30H33BF2N4O4 |
| Molecular Weight | 562.42 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
A thiol-reactive fluorescent probe for cysteine labeling with bright orange fluorescence. Buy fluorescent labeling reagents online for bioconjugation and protein imaging.
Chemical Information
Product Specification
Application
| Synonyms | EverFluor TMR C5-maleimide |
| Appearance | Solid Powder |
| Excitation | 494 |
| Emission | 520 |
BODIPY TMR C5-maleimide is a fluorescent dye that is part of the BODIPY (boron-dipyrromethene) family, specifically designed for labeling biomolecules in various biological and biochemical applications. The dye has a tetramethylrhodamine (TMR) fluorophore, which emits in the red region of the spectrum, making it ideal for multi-color fluorescence experiments. The maleimide group attached to the C5 position of the BODIPY dye allows for selective conjugation to thiol groups, such as those present in cysteine residues of proteins, enabling specific labeling of biomolecules.
One key application of BODIPY TMR C5-maleimide is in protein labeling. Due to its maleimide group, it can specifically bind to thiol groups in proteins, particularly those containing cysteine residues. This allows for targeted labeling and tracking of proteins in complex biological systems. This application is widely used in protein structure-function studies, protein interaction mapping, and cellular localization experiments. Researchers use the dye to study the behavior and interaction of proteins within cells and tissues.
Another significant application of BODIPY TMR C5-maleimide is in real-time monitoring of cellular processes. The dye's strong fluorescence and stability make it ideal for live-cell imaging. By labeling proteins or other biomolecules with BODIPY TMR C5-maleimide, researchers can visualize dynamic cellular events, such as protein trafficking, receptor internalization, and vesicle transport. This is especially useful in studies of signal transduction and cellular responses to stimuli.
BODIPY TMR C5-maleimide is also employed in flow cytometry and fluorescence-activated cell sorting (FACS) for cell analysis. The red fluorescence emitted by the dye allows for clear discrimination in multi-color assays, enabling researchers to analyze the expression levels and distribution of labeled proteins or other cellular components. This application is valuable in immunology, cancer research, and cell biology for sorting and analyzing specific cell populations.
Additionally, BODIPY TMR C5-maleimide is used in assay development for drug screening. The dye can be used to track the binding of small molecules to target proteins, especially those that involve cysteine residues in their active sites. By monitoring fluorescence changes upon binding, researchers can identify potential drug candidates that modify protein activity or inhibit specific interactions. This application is useful in high-throughput screening of therapeutic compounds in drug discovery.
Recommended Services
Recommended Articles
- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
- Fluorescent Probes: Definition, Structure, Types and Application
- Fluorescent Dyes: Definition, Mechanism, Types and Application
- Coumarin Dyes: Definition, Structure, Benefits, Synthesis and Uses
- BODIPY Dyes: Definition, Structure, Synthesis and Uses
- Cyanine Dyes: Definition, Structure, Types and Uses
- Fluorescein Dyes: Definition, Structure, Synthesis and Uses
- Rhodamine Dyes: Definition, Structure, Uses, Excitation and Emission
- Unlocking the Power of Fluorescence Imaging: A Comprehensive Guide
- Cell Imaging: Definitions, Systems, Protocols, Dyes, and Applications
- Lipid Staining: Definition, Principles, Methods, Dyes, and Uses
- Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses
- Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses
- DNA Staining: Definition, Procedures, Benefits, Dyes and Uses
- Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
- Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
- Fluorescent Labeling: Definition, Principles, Types and Applications
Recommended Products
Online Inquiry
