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BODIPY
BODIPY FL N-(2-Aminoethyl)Maleimide

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BODIPY FL N-(2-Aminoethyl)Maleimide

Catalog Number	F01-0169
Category	BODIPY
Molecular Formula	C20H21BF2N4O3
Molecular Weight	414.22

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Product Introduction

BODIPY FL N-(2-Aminoethyl)Maleimide is a thiol-reactive, green-fluorescent dye used to create bioconjugates with peptides, proteins, and thiol-modified nucleotides at pH less than 7.5. It is relatively insensitive to environment and pH changes.

Chemical Information
Application

Synonyms	EverFluor FL N-(2-Aminoethyl)Maleimide; BODIPY FL maleimide
Appearance	Solid Powder

BODIPY FL N-(2-Aminoethyl)Maleimide, a highly versatile fluorescent dye widely utilized in biological and chemical research, boasts a multitude of applications.

Protein Labeling: BODIPY FL N-(2-Aminoethyl)Maleimide stands out as a prime choice for labeling proteins, simplifying the exploration of their cellular localization, trafficking patterns, and interactions. The maleimide group establishes stable covalent bonds with thiol groups in proteins, allowing researchers to visualize protein dynamics using fluorescence microscopy with remarkable sensitivity and resolution.

Cellular Imaging: Embarking on live-cell imaging endeavors, researchers harness the exceptional photostability and brightness of this dye to monitor intracellular processes and cellular responses to various treatments. Its superior capabilities are ideal for long-term and time-lapse imaging experiments, enabling real-time analysis of cell migration, division, and protein-protein interactions.

Lipid Research: Venturing into lipid studies, BODIPY FL N-(2-Aminoethyl)Maleimide serves as a guiding light for investigating lipid membranes and lipid-protein interactions. By integrating this dye into lipid molecules, scientists can delve into lipid distribution and dynamics within cells, shedding light on membrane organization, lipid trafficking, and the impacts of pharmaceutical compounds on membrane properties.

Flow Cytometry: Illuminating the path in flow cytometry, this fluorescent dye plays a crucial role in labeling and quantifying cells or particles based on their biochemical and physical characteristics. Conjugating BODIPY FL N-(2-Aminoethyl)Maleimide to antibodies or other targeting molecules empowers researchers to pinpoint and segregate specific cell populations, a pivotal step for applications like immunophenotyping, cell cycle analysis, and detection of rare cellular events.