
BODIPY FL Ethylamine
Catalog Number | F01-0175 |
Category | BODIPY |
Molecular Formula | C15H20BF2N3 |
Molecular Weight | 291.15 |
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Product Introduction
BODIPY FL Ethylamine is a fluorescent dye that reversibly reacts with aldehyde/ketone to yield a Schiff base that can be reduced to form a stable amine derviative using sodium borohydride or sodium cyanoborohydride. BODIPY FL is non-ionic, relatively insensitive to environment and pH changes.
Chemical Information |
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Synonyms | EverFluor FL Ethylamine |
Appearance | Solid Powder |
- Product Specification
- Application
Excitation | 494 |
Emission | 522 |
BODIPY FL Ethylamine is a fluorescent dye that is widely used in various scientific research applications due to its exceptional photostability and bright fluorescence. Here are some key applications of BODIPY FL Ethylamine:
Fluorescence Microscopy: BODIPY FL Ethylamine is often used in fluorescence microscopy to visualize cellular structures and processes. Its high brightness and photostability make it ideal for long-term imaging experiments, allowing researchers to track dynamic changes within cells. By conjugating BODIPY FL Ethylamine to specific biomolecules, scientists can achieve precise labeling and localization of target proteins or organelles.
Flow Cytometry: In flow cytometry, BODIPY FL Ethylamine serves as a vital fluorescent marker for cell sorting and analysis. Its compatibility with various instruments enables the simultaneous detection of multiple parameters in individual cells. Researchers use this dye to measure cell viability, proliferation, and to identify distinct cell populations based on fluorescence intensity.
Bioconjugation: BODIPY FL Ethylamine can be chemically linked to a variety of biomolecules through covalent bonds, enabling its use in bioconjugation techniques. Researchers frequently use this dye to label antibodies, peptides, or nucleic acids for subsequent detection in assays. This application is crucial for developing diagnostic tools and biosensors that rely on fluorescence-based detection methods.
Live Cell Imaging: The properties of BODIPY FL Ethylamine make it well-suited for live cell imaging studies where maintaining cell viability is crucial. Its hydrophobic nature allows it to easily incorporate into cell membranes, providing insights into membrane dynamics and interactions. Researchers can monitor real-time biochemical processes and cellular responses with minimal photobleaching and cytotoxicity.
Applications of Fluorescent Probes & Dyes
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