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BODIPY 630/650 C2-Maleimide
| Catalog Number | F01-0171 |
| Category | BODIPY |
| Molecular Formula | C29H23BN4O4S |
| Molecular Weight | 572.39 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 630/650 C2-Maleimide is a thiol-reactive fluorophore that features a boron-dipyrromethene (BODIPY) core, known for its exceptional photostability and sharp emission peaks. It contains a maleimide functional group, enabling selective conjugation to sulfhydryl groups on proteins and peptides, making it useful for targeted labeling applications. Exhibiting a red-shifted fluorescence with excitation and emission maxima around 630 nm and 650 nm respectively, it is commonly utilized in fluorescence microscopy, flow cytometry, and other bioimaging techniques requiring precise molecular tracking and intracellular localization.
Chemical Information
Application
Chemical Information
| Synonyms | EverFluor 630/650 C2-maleimide |
| Appearance | Solid Powder |
Application
BODIPY 630/650 C2-Maleimide is a fluorescent dye known for its applications in a variety of scientific research fields. Here are some key applications of BODIPY 630/650 C2-Maleimide:
Fluorescent Labeling: BODIPY 630/650 C2-Maleimide is frequently used as a fluorescent label in biological imaging studies. Its ability to covalently bind to thiol groups on proteins makes it ideal for tagging and tracing protein interactions within cells. This property is crucial for visualizing cellular processes such as protein trafficking, localization, and interactions.
Flow Cytometry: In flow cytometry applications, BODIPY 630/650 C2-Maleimide serves as a reliable dye for labeling and analyzing cell populations. The dye’s bright fluorescence and photostability aid in distinguishing between different cell types or states. It allows researchers to perform multi-color analyses by pairing with other fluorescent markers, enhancing the breadth of cellular assays.
FRET (Förster Resonance Energy Transfer) Studies: BODIPY 630/650 C2-Maleimide is utilized in FRET experiments to study molecular interactions and conformational changes. Its spectral properties enable effective pairing with other fluorophores, facilitating the investigation of dynamic biological processes and protein-protein interactions at the nanoscale. This knowledge is pivotal for understanding the mechanistic details of complex biological systems.
Protein Research: The dye is also employed in protein research, notably in electrophoresis and Western blotting techniques. Its binding to proteins provides a means for the sensitive detection of proteins in gels and membranes. Researchers leverage its fluorescent properties to quantify protein expression levels, presence, or modifications, contributing to detailed protein analyses and discoveries.
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