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Fluorescent Dyes
BODIPY
BODIPY 581/591 SE

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BODIPY 581/591 SE

Catalog Number	F01-0183
Category	BODIPY
Molecular Formula	C26H22BF2N3O4
Molecular Weight	489.28

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Product Introduction

BODIPY 581/591 SE is an orange-fluorescent dye that is reactive with primary amines on biomolecules such as peptides, proteins, modified nucleotides and biopolymers. It is non-ionic, and relatively insensitive to environment and pH changes.

Chemical Information
Product Specification
Application

Synonyms	EverFluor 581/591 SE
Appearance	Solid Powder

Excitation	494
Emission	520

BODIPY 581/591 SE (Succinimidyl Ester) is a fluorescent dye that belongs to the BODIPY (boron-dipyrromethene) family, known for its intense fluorescence in the red region of the spectrum (around 591 nm). The succinimidyl ester group enables the dye to covalently link to amine-containing biomolecules, such as proteins, peptides, and nucleic acids, facilitating its use in various bioconjugation applications. This dye is highly photostable, making it ideal for long-term imaging and analysis in biological systems.

One primary application of BODIPY 581/591 SE is in fluorescence labeling for microscopy and flow cytometry. Its bright and stable fluorescence allows researchers to track the behavior of proteins and other biomolecules in live cells and tissues. By conjugating the dye to antibodies or peptides, researchers can study specific protein localization, cellular trafficking, and interactions in real-time, providing valuable insights into cellular dynamics and disease mechanisms.

BODIPY 581/591 SE is also widely used in immunoassays, such as enzyme-linked immunosorbent assays (ELISA) and Western blotting. Its strong red fluorescence enhances the sensitivity and detection capabilities of these assays, making it easier to visualize and quantify the presence of target molecules. This application is critical in diagnostics and biomedical research, where precise detection of specific biomarkers is essential for disease detection and understanding molecular pathways.

In addition, BODIPY 581/591 SE is utilized in the development of molecular probes for studying protein-protein interactions. By conjugating the dye to proteins or peptides, researchers can visualize and study how molecules interact within cells or in vitro. This application is important in drug discovery and the study of cellular signaling pathways, where understanding molecular interactions can lead to the development of targeted therapies.

Lastly, BODIPY 581/591 SE is employed in the creation of biosensors. Its fluorescent properties enable the design of sensors that detect specific molecules or environmental changes, such as pH or ion concentrations, in living cells or complex biological samples. These sensors can be used in a variety of research fields, including environmental monitoring, pharmaceutical development, and metabolic studies.