
BODIPY 558/568 SE
| Catalog Number | F01-0191 |
| Category | BODIPY |
| Molecular Formula | C20H16BF2N3O4S |
| Molecular Weight | 443.23 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 558/568 SE is a fluorescent dye belonging to the boron-dipyrromethene (BODIPY) class, known for its exceptional photostability and sharp spectral properties. It features succinimidyl ester (SE) reactive groups that enable efficient conjugation to primary amines on proteins, peptides, or other biomolecules, facilitating its use in fluorescence microscopy and flow cytometry applications. With excitation and emission maxima in the visible spectrum, BODIPY 558/568 SE supports diverse imaging workflows and is incorporated into assays requiring precise molecular tracking and visualization.
Chemical Information
Product Specification
Application
Chemical Information
| Synonyms | EverFluor 558/568 SE; BODIPY 558/568 NHS Ester |
| Appearance | Solid Powder |
Product Specification
| Excitation | 496 |
| Emission | 520 |
Application
BODIPY 558/568 SE is a fluorescent dye commonly used in scientific research for labeling and detection purposes. Here are some key applications of BODIPY 558/568 SE:
Fluorescence Microscopy: BODIPY 558/568 SE is frequently utilized in fluorescence microscopy to label proteins, nucleic acids, and other biomolecules within cells. Its exceptional photostability and bright fluorescence enable detailed imaging of cellular structures and dynamic processes. Researchers can track the localization and movement of biomolecules in live cells, facilitating the study of cellular biology.
Flow Cytometry: In flow cytometry, BODIPY 558/568 SE is used as a fluorescent marker to identify and quantify specific cell populations. By conjugating the dye to antibodies or ligands, scientists can analyze cell surface proteins and intracellular molecules. This application is crucial for immunophenotyping, cell sorting, and measuring cellular responses in various research and clinical settings.
Protein Labeling: BODIPY 558/568 SE is often employed in labeling proteins for biochemical and biophysical analyses. Its high solubility and compatibility make it ideal for conjugation with proteins without altering their function. This enables the investigation of protein-protein interactions, protein dynamics, and enzyme activity in detail, enhancing our understanding of molecular mechanisms.
Live Cell Imaging: With its low cytotoxicity and robust fluorescence, BODIPY 558/568 SE is an excellent choice for live cell imaging studies. Researchers can visualize cellular processes in real-time, such as organelle movement, signaling pathways, and cell division. This application is vital for understanding dynamic events within living cells and assessing the impact of various treatments on cellular behavior.
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