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Fluorescent Dyes
BODIPY
BODIPY 558/568 SE

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BODIPY 558/568 SE

Catalog Number	F01-0191
Category	BODIPY
Molecular Formula	C20H16BF2N3O4S
Molecular Weight	443.23

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Product Introduction

BODIPY 558/568 SE is a yellow-fluorescent dye that is reactive with primary amines on biomolecules such as peptides, proteins, modified nucleotides and biopolymers. It is non-ionic, and relatively insensitive to environment and pH changes.

Chemical Information
Product Specification
Application

Synonyms	EverFluor 558/568 SE; BODIPY 558/568 NHS Ester
Appearance	Solid Powder

Excitation	496
Emission	520

BODIPY 558/568 SE is a fluorescent dye commonly used in scientific research for labeling and detection purposes. Here are some key applications of BODIPY 558/568 SE:

Fluorescence Microscopy: BODIPY 558/568 SE is frequently utilized in fluorescence microscopy to label proteins, nucleic acids, and other biomolecules within cells. Its exceptional photostability and bright fluorescence enable detailed imaging of cellular structures and dynamic processes. Researchers can track the localization and movement of biomolecules in live cells, facilitating the study of cellular biology.

Flow Cytometry: In flow cytometry, BODIPY 558/568 SE is used as a fluorescent marker to identify and quantify specific cell populations. By conjugating the dye to antibodies or ligands, scientists can analyze cell surface proteins and intracellular molecules. This application is crucial for immunophenotyping, cell sorting, and measuring cellular responses in various research and clinical settings.

Protein Labeling: BODIPY 558/568 SE is often employed in labeling proteins for biochemical and biophysical analyses. Its high solubility and compatibility make it ideal for conjugation with proteins without altering their function. This enables the investigation of protein-protein interactions, protein dynamics, and enzyme activity in detail, enhancing our understanding of molecular mechanisms.

Live Cell Imaging: With its low cytotoxicity and robust fluorescence, BODIPY 558/568 SE is an excellent choice for live cell imaging studies. Researchers can visualize cellular processes in real-time, such as organelle movement, signaling pathways, and cell division. This application is vital for understanding dynamic events within living cells and assessing the impact of various treatments on cellular behavior.