BODIPY 530/550 SE

BODIPY 530/550 SE is a fluorescent dye with an excitation maximum around 530 nm and an emission maximum around 550 nm, giving it a bright green-yellow fluorescence. This dye is part of the BODIPY (boron-dipyrromethene) family, known for its excellent brightness, photostability, and resistance to photobleaching. Its versatility makes it a valuable tool in various biological and chemical applications, particularly in fluorescence microscopy and flow cytometry.

One of the key applications of BODIPY 530/550 SE is in cellular labeling for fluorescence imaging. It can be easily conjugated to proteins, antibodies, or other biomolecules, allowing researchers to track their movement and interactions in living cells. This makes it invaluable for studies in protein localization, intracellular trafficking, and cellular signaling, aiding in the visualization of dynamic biological processes.

Another important use of BODIPY 530/550 SE is in multicolor flow cytometry. The dye’s distinct emission spectrum allows it to be used in conjunction with other fluorophores for simultaneous analysis of multiple cell markers. This capability is particularly useful in immunophenotyping and cancer research, where complex mixtures of cell populations need to be analyzed for specific markers and behaviors.

BODIPY 530/550 SE is also utilized in high-throughput screening (HTS) assays, particularly in drug discovery. Its fluorescence properties are used to monitor molecular interactions, enzyme activities, or cellular responses in large-scale assays. The dye’s sensitivity and stability make it ideal for screening compounds that affect cellular functions or bind to specific targets, helping researchers identify potential drug candidates.

Lastly, BODIPY 530/550 SE is used in chemical sensing and environmental monitoring. The dye can be incorporated into sensor systems designed to detect changes in pH, ions, or other chemical signals. Its fluorescence shifts upon interaction with specific analytes, making it an effective tool for real-time monitoring of chemical reactions, environmental conditions, or biological processes.