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Fluorescent Dyes
BODIPY
BODIPY 530/550 SE

What We Offer

BODIPY 530/550 SE

Catalog Number	F01-0187
Category	BODIPY
Molecular Formula	C28H22BF2N3O4
Molecular Weight	513.3

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Product Introduction

BODIPY 530/550 SE is an orange-fluorescent dye that is reactive with primary amines on peptides, proteins, modified nucleotides, biopolymers and other amine-containing biomolecules. It is non-ionic, and relatively insensitive to environment and pH changes.

Chemical Information
Product Specification
Application

Synonyms	EverFluor 530/550 SE; BODIPY 530/550 NHS Ester
Appearance	Solid Powder

Excitation	501
Emission	523

BODIPY 530/550 SE is a fluorescent dye with an excitation maximum around 530 nm and an emission maximum around 550 nm, giving it a bright green-yellow fluorescence. This dye is part of the BODIPY (boron-dipyrromethene) family, known for its excellent brightness, photostability, and resistance to photobleaching. Its versatility makes it a valuable tool in various biological and chemical applications, particularly in fluorescence microscopy and flow cytometry.

One of the key applications of BODIPY 530/550 SE is in cellular labeling for fluorescence imaging. It can be easily conjugated to proteins, antibodies, or other biomolecules, allowing researchers to track their movement and interactions in living cells. This makes it invaluable for studies in protein localization, intracellular trafficking, and cellular signaling, aiding in the visualization of dynamic biological processes.

Another important use of BODIPY 530/550 SE is in multicolor flow cytometry. The dye’s distinct emission spectrum allows it to be used in conjunction with other fluorophores for simultaneous analysis of multiple cell markers. This capability is particularly useful in immunophenotyping and cancer research, where complex mixtures of cell populations need to be analyzed for specific markers and behaviors.

BODIPY 530/550 SE is also utilized in high-throughput screening (HTS) assays, particularly in drug discovery. Its fluorescence properties are used to monitor molecular interactions, enzyme activities, or cellular responses in large-scale assays. The dye’s sensitivity and stability make it ideal for screening compounds that affect cellular functions or bind to specific targets, helping researchers identify potential drug candidates.

Lastly, BODIPY 530/550 SE is used in chemical sensing and environmental monitoring. The dye can be incorporated into sensor systems designed to detect changes in pH, ions, or other chemical signals. Its fluorescence shifts upon interaction with specific analytes, making it an effective tool for real-time monitoring of chemical reactions, environmental conditions, or biological processes.