![(E)-10-(4-(5,5-dimethyl-1,3,2-dioxaborinan-2-yl)phenyl)-3-(4-(dimethylamino)styryl)-5,5-difluoro-1,7,9-trimethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide](https://resource.bocsci.com/structure/949108-72-9.gif)
Inquiry Basket
BODIPY 530/550 IA
| Catalog Number | F01-0170 |
| Category | BODIPY |
| Molecular Formula | C28H26BF2IN4O2 |
| Molecular Weight | 626.24 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 530/550 IA is a fluorescent dye belonging to the boron-dipyrromethene class, recognized for its strong absorption and high fluorescence quantum yield in the green spectral region. This compound features a conjugated aromatic system that facilitates efficient energy transfer, making it suitable for fluorescence resonance energy transfer (FRET) applications and as a reporter molecule in various bioimaging assays. Its reactive functionality allows for covalent conjugation with biomolecules, supporting its use in protein labeling and intracellular tracking within fluorescence microscopy workflows.
Chemical Information
Application
Chemical Information
| Synonyms | EverFluor 530/550 IA; N-(4,4-difluoro-5,7-diphenyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl)-N'-Iodoacetylethylenediamine |
| Appearance | Solid Powder |
Application
BODIPY 530/550 IA is a fluorescent dye widely used in various scientific applications due to its bright fluorescence and photostability. Here are some key applications of BODIPY 530/550 IA:
Fluorescence Microscopy: BODIPY 530/550 IA is employed in fluorescence microscopy techniques to label and visualize cellular structures and molecules. Its distinct fluorescent properties allow researchers to track dynamic biological processes in living cells with high resolution.
Flow Cytometry: In flow cytometry, BODIPY 530/550 IA is used to stain and differentiate cell populations based on their fluorescence characteristics. The dye’s emission spectrum enables the detection of specific cell markers in a multi-color panel. This enhances the ability to analyze complex samples such as blood or tissue and perform detailed immunophenotyping.
Confocal Laser Scanning Microscopy: BODIPY 530/550 IA is valuable in confocal laser scanning microscopy for achieving optical sectioning and 3D reconstructions of specimens. The dye’s compatibility with confocal imaging allows for the capture of high-contrast, detailed images without background interference. This technique is essential for examining intricate biological structures and understanding spatial relationships within cells and tissues.
Protein Labeling: BODIPY 530/550 IA is used for labeling proteins in biochemical assays to study protein-protein interactions and conformational changes. This dye provides a stable and intense signal that aids in real-time monitoring of proteins in solution or immobilized on surfaces. Such labeling is crucial for elucidating protein function and dynamics in various research and diagnostic applications.
Recommended Services
Recommended Articles
- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
- Fluorescent Probes: Definition, Structure, Types and Application
- Fluorescent Dyes: Definition, Mechanism, Types and Application
- Coumarin Dyes: Definition, Structure, Benefits, Synthesis and Uses
- Unlocking the Power of Fluorescence Imaging: A Comprehensive Guide
- Cell Imaging: Definitions, Systems, Protocols, Dyes, and Applications
- Lipid Staining: Definition, Principles, Methods, Dyes, and Uses
- Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses
- Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses
Recommended Products
Online Inquiry
