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Fluorescent Dyes
BODIPY
BODIPY 530/550 IA

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BODIPY 530/550 IA

Catalog Number	F01-0170
Category	BODIPY
Molecular Formula	C28H26BF2IN4O2
Molecular Weight	626.24

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Product Introduction

BODIPY 530/550 IA is a thiol-reactive, orange-fluorescent dye used to create bioconjugates with peptides, proteins, and thiol-modified nucleotides at pH less than 7.5. It is relatively insensitive to environment and pH changes.

Chemical Information
Application

Synonyms	EverFluor 530/550 IA; N-(4,4-difluoro-5,7-diphenyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl)-N'-Iodoacetylethylenediamine
Appearance	Solid Powder

BODIPY 530/550 IA is a fluorescent dye widely used in various scientific applications due to its bright fluorescence and photostability. Here are some key applications of BODIPY 530/550 IA:

Fluorescence Microscopy: BODIPY 530/550 IA is employed in fluorescence microscopy techniques to label and visualize cellular structures and molecules. Its distinct fluorescent properties allow researchers to track dynamic biological processes in living cells with high resolution.

Flow Cytometry: In flow cytometry, BODIPY 530/550 IA is used to stain and differentiate cell populations based on their fluorescence characteristics. The dye’s emission spectrum enables the detection of specific cell markers in a multi-color panel. This enhances the ability to analyze complex samples such as blood or tissue and perform detailed immunophenotyping.

Confocal Laser Scanning Microscopy: BODIPY 530/550 IA is valuable in confocal laser scanning microscopy for achieving optical sectioning and 3D reconstructions of specimens. The dye’s compatibility with confocal imaging allows for the capture of high-contrast, detailed images without background interference. This technique is essential for examining intricate biological structures and understanding spatial relationships within cells and tissues.

Protein Labeling: BODIPY 530/550 IA is used for labeling proteins in biochemical assays to study protein-protein interactions and conformational changes. This dye provides a stable and intense signal that aids in real-time monitoring of proteins in solution or immobilized on surfaces. Such labeling is crucial for elucidating protein function and dynamics in various research and diagnostic applications.