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BODIPY 505/515 | CAS 21658-70-8
| Catalog Number | F01-0045 |
| Category | BODIPY |
| Molecular Formula | C13H15BF2N2 |
| Molecular Weight | 248.1 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 505/515 is a lipophilic fluorescent probe that localizes to intracellular lipid bodies and can be used to label lipid droplets in live and fixed cells. BODIPY 505/515 has been used for electron, epifluorescent, and confocal microscopy, as well as flow cytometry applications in various algae species.
Chemical Information
Product Specification
Application
| Synonyms | 1,3,5,7-Tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; (T-4)-[2-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-3,5-dimethyl-1H-pyrrolato-κN]difluoro-boron |
| Purity | ≥98% |
| Canonical SMILES | [B-]1(N2C(=CC(=C2C=C3[N+]1=C(C=C3C)C)C)C)(F)F |
| InChI | InChI=1S/C13H15BF2N2/c1-8-5-10(3)17-12(8)7-13-9(2)6-11(4)18(13)14(17,15)16/h5-7H,1-4H3 |
| InChIKey | TWYZTUZOEQTCOO-UHFFFAOYSA-N |
| Appearance | Solid Powder |
| Excitation | 505 nm |
| Emission | 515 nm |
| Storage | Store at -20°C |
BODIPY 505/515 is a fluorescent dye widely used in various bioscience applications due to its bright fluorescence and versatility. Here are some key applications of BODIPY 505/515:
Live Cell Imaging: BODIPY 505/515 is extensively used in live cell imaging to visualize cellular structures and dynamics. Its photostability and bright fluorescence make it ideal for long-term imaging studies. Researchers use it to label proteins, lipids, and other cellular components to track their location and movement within living cells.
Flow Cytometry: In flow cytometry, BODIPY 505/515 is employed as a fluorescent marker to analyze the properties of individual cells within a heterogeneous population. It enables the detection and quantification of specific cell surface markers or intracellular molecules. This application is crucial for applications such as immunophenotyping, cell sorting, and cell cycle analysis.
Fluorescence Microscopy: BODIPY 505/515 is used in fluorescence microscopy to stain biological samples and visualize microenvironments in high detail. Its compatibility with various types of microscopes, such as confocal and wide-field, allows for versatile imaging applications. This dye is instrumental in studying detailed cellular architecture and interactions with high-resolution images.
Assay Development: BODIPY 505/515 is a popular choice in developing biochemical and cellular assays for research and diagnostic purposes. Its unique spectral properties enhance the sensitivity and specificity of assays such as enzyme activity assays and binding studies. Researchers utilize this dye to develop robust and reproducible assays for various biological research and clinical diagnostics.
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