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BODIPY
BODIPY 493/503 Methyl Bromide

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BODIPY 493/503 Methyl Bromide

Catalog Number	F01-0168
Category	BODIPY
Molecular Formula	C14H16BBrF2N2
Molecular Weight	341

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Product Introduction

BODIPY 493/503 Methyl Bromide is a cell-permeant, green-fluorescent dye for long-term staining of live cells. It is well retained in the cell and the methyl bromide group reacts with internal cell components, presumably glutathione, yielding membrane-impermeant, green-fluorescent adducts.

Chemical Information
Application

Synonyms	CellHunt Green 493/503 Methyl Bromide; EverFluor 493/503 Methyl Bromide; 8-Bromomethyl-4,4-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a,4a-Diaza-s-Indacene
Appearance	Solid Powder

BODIPY 493/503 methyl bromide, a fluorescent dye known for its exceptional stability and photophysical characteristics, finds widespread utility across various fields. Here are four key applications of BODIPY 493/503 methyl bromide:

Fluorescence Microscopy: Embraced by researchers in fluorescence microscopy, BODIPY 493/503 methyl bromide emerges as a standout choice for visualizing intricate cellular structures and dynamic biological processes. Its luminous fluorescence and enduring photostability render it an optimal candidate for protracted imaging endeavors, allowing scientists to intricately label specific proteins or lipids and closely scrutinize the ever-evolving cellular dynamics within live or fixed samples.

Flow Cytometry: A pivotal tool in flow cytometry, BODIPY 493/503 methyl bromide serves as a potent fluorescent marker essential for precise cell sorting and in-depth analysis. Its robust fluorescence capabilities facilitate the accurate identification and quantification of labeled cells, thereby enhancing the trustworthiness of experimental findings. This application plays a critical role in diverse studies encompassing immune profiling, cell cycle investigations, and the meticulous sorting of individual cells.

Lipophilicity Studies: Widely embraced in the realm of lipophilicity research, BODIPY 493/503 methyl bromide stands out for its unique ability to seamlessly integrate into lipid membranes. By tagging lipid components with this dye, researchers gain valuable insights into lipid distribution and dynamics within varied biological systems. This aids in unraveling the intricate organization of cell membranes and sheds light on the pivotal role played by lipids in driving cellular processes.

Chemical Sensing: Beyond its conventional uses, this dye finds versatile applications in cutting-edge chemical sensing technologies owing to its distinctive fluorescence alterations upon encountering specific analytes. BODIPY 493/503 methyl bromide sensors exhibit exceptional sensitivity and specificity in detecting ions, small molecules, and environmental shifts.