BDY FL, SE

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BDY FL, SE

BDY FL, SE | 146616-66-2

Catalog Number R01-0009
Category BODIPY
Molecular Formula C18H18BF2N3O4
Molecular Weight 389.166
Catalog Number Size Price Quantity
R01-0009 50 mg $519

Product Introduction

BODIPY dyes are used to generate fluorescent conjugates of proteins, nucleotides, oligonucleotides and dextrans, as well as to prepare fluorescent enzyme substrates, fatty acids, phospholipids, lipopolysaccharides, receptor ligands and polystyrene microspheres.

Chemical Information

Synonyms 3-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)-5,5-difluoro-7,9-dimethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide; 7-(3-((2,5-Dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)-5,5-difluoro-1,3-dimethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide; 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionic Acid, Succinimidyl Ester; BDP FL NHS ester
Purity NMR 1H, HPLC-MS (95+%)
Canonical SMILES [B-]1(N2C(=CC(=C2C=C3[N+]1=C(C=C3)CCC(=O)ON4C(=O)CCC4=O)C)C)(F)F
InChI InChI=1S/C18H18BF2N3O4/c1-11-9-12(2)22-15(11)10-14-4-3-13(23(14)19(22,20)21)5-8-18(27)28-24-16(25)6-7-17(24)26/h3-4,9-10H,5-8H2,1-2H3
InChI Key SDVMGNSTOAJONW-UHFFFAOYSA-N
Solubility Good in organic solvents (DMF, DMSO, dichlromethane), limited in water
Appearance orange solid
  • Product Specification
  • Application
ε, L⋅mol-1⋅cm-1 92000
Fluorescence Quantum Yield 0.97
Excitation 502 nm
Emission 510 nm
Storage Store at -20°C

BDY FL, SE is a fluorescent dye commonly used in various bioscience applications due to its high sensitivity and specificity. Here are some key applications of BDY FL, SE:

Cell Imaging: BDY FL, SE is widely used in cell imaging to stain and visualize specific structures or proteins within cells. Its high fluorescence intensity allows for detailed imaging of cellular components, such as membranes, organelles, and cytoskeletal elements. This application is essential for studying cell morphology, dynamics, and interactions in both live and fixed cells.

Flow Cytometry: BDY FL, SE is frequently employed in flow cytometry to label cell populations for various analyses. By conjugating BDY FL, SE to antibodies or other markers, cells can be accurately identified and quantified based on fluorescence intensity. This technique is vital for immunophenotyping, cell sorting, and studying cell cycle dynamics.

Molecular Biology: In molecular biology, BDY FL, SE is often used to label nucleic acids for applications such as fluorescence in situ hybridization (FISH) and quantitative PCR. The dye can be covalently attached to DNA or RNA probes, allowing for the detection and quantification of specific sequences. This facilitates the study of gene expression, chromosomal abnormalities, and microbial detection.

Protein Studies: BDY FL, SE can be used to label proteins for various biochemical and biophysical experiments. By conjugating the dye to proteins, researchers can study protein localization, interactions, and function using fluorescence-based techniques. This application is crucial for understanding protein dynamics, pathway analysis, and drug discovery.

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