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Ac-LETD-AFC | CAS 210345-02-1
| Catalog Number | A18-0010 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C31H38F3N5O12 |
| Molecular Weight | 729.7 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Ac-LETD-AFC is a fluorogenic substrate commonly used in biochemical assays to monitor caspase activity. It features a tetrapeptide sequence linked to 7-amino-4-trifluoromethylcoumarin (AFC), a fluorophore that exhibits strong fluorescence upon cleavage by caspases. This compound is widely employed in apoptosis research, where it serves as an indicator of protease activity through its fluorescence emission, enabling the study of cellular pathways and enzyme kinetics.
Chemical Information
Product Specification
Application
Chemical Information
| Synonyms | N-Acetyl-Leu-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin; N-acetyl-L-leucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine |
| Purity | ≥95% |
| SMILES | CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C |
| InChI | InChI=1S/C31H38F3N5O12/c1-13(2)9-20(35-15(4)41)29(49)37-19(7-8-23(42)43)27(47)39-26(14(3)40)30(50)38-21(12-24(44)45)28(48)36-16-5-6-17-18(31(32,33)34)11-25(46)51-22(17)10-16/h5-6,10-11,13-14,19-21,26,40H,7-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,38,50)(H,39,47)(H,42,43)(H,44,45)/t14-,19+,20+,21+,26+/m1/s1 |
| InChIKey | KTZOWCXXAYFIFT-XSSXFGQWSA-N |
| Appearance | Solid Powder |
Product Specification
| Excitation | 400 nm |
| Emission | 505 nm |
| Storage | Store at -20°C |
Application
Ac-LETD-AFC is a synthetic peptide fluorogenic substrate widely used in bioscience research to measure protease activity, particularly caspase-8. Here are some key applications of Ac-LETD-AFC:
Apoptosis Studies: Ac-LETD-AFC is commonly used in apoptosis research to detect caspase-8 activity, a key enzyme involved in the extrinsic pathway of apoptosis. By monitoring the fluorescence released upon cleavage of the substrate, researchers can quantify caspase-8 activity in cell lysates. This can help in understanding the mechanisms of cell death and the role of caspase-8 in various diseases.
Drug Screening: In pharmaceutical research, Ac-LETD-AFC is employed in high-throughput screening assays to identify potential inhibitors or activators of caspase-8. Compounds that modulate caspase-8 activity can be identified by their effect on the fluorescence signal. This is valuable for the development of new therapeutic agents aimed at manipulating apoptosis for treating diseases such as cancer.
Toxicology Studies: Ac-LETD-AFC is also used in toxicology to evaluate the cytotoxic effects of chemicals and drug candidates on cells. By measuring caspase-8 activation, one can assess the extent of apoptosis induced by toxic substances. This aids in determining the potential safety and adverse effects of new pharmaceuticals or environmental toxins.
Basic Research: Beyond applied sciences, Ac-LETD-AFC serves as an important tool in basic research to study the intricacies of protease function and regulation. Researchers can use it to analyze the activity of purified caspase-8 and other related proteases in vitro. This contributes to a deeper understanding of protease biology and the development of assays for other proteolytic enzymes.
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