![{[Name]}](https://resource.bocsci.com/structure/18997-57-4.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/881005-91-0.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/76204-02-9.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/126938-14-5.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/19088-73-4.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/206443-06-3.gif?v=2025011501)
Inquiry Basket
Ac-LETD-AFC | CAS 210345-02-1
| Catalog Number | A18-0010 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C31H38F3N5O12 |
| Molecular Weight | 729.7 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Ac-LETD-AFC is a fluorogenic substrate for caspase-8. Caspase activity can be quantified by fluorescent detection of free AFC (7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.
Chemical Information
Product Specification
Application
| Synonyms | N-Acetyl-Leu-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin; N-acetyl-L-leucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine |
| Purity | ≥95% |
| Canonical SMILES | CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C |
| InChI | InChI=1S/C31H38F3N5O12/c1-13(2)9-20(35-15(4)41)29(49)37-19(7-8-23(42)43)27(47)39-26(14(3)40)30(50)38-21(12-24(44)45)28(48)36-16-5-6-17-18(31(32,33)34)11-25(46)51-22(17)10-16/h5-6,10-11,13-14,19-21,26,40H,7-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,38,50)(H,39,47)(H,42,43)(H,44,45)/t14-,19+,20+,21+,26+/m1/s1 |
| InChIKey | KTZOWCXXAYFIFT-XSSXFGQWSA-N |
| Appearance | Solid Powder |
| Excitation | 400 nm |
| Emission | 505 nm |
| Storage | Store at -20°C |
Ac-LETD-AFC is a synthetic peptide fluorogenic substrate widely used in bioscience research to measure protease activity, particularly caspase-8. Here are some key applications of Ac-LETD-AFC:
Apoptosis Studies: Ac-LETD-AFC is commonly used in apoptosis research to detect caspase-8 activity, a key enzyme involved in the extrinsic pathway of apoptosis. By monitoring the fluorescence released upon cleavage of the substrate, researchers can quantify caspase-8 activity in cell lysates. This can help in understanding the mechanisms of cell death and the role of caspase-8 in various diseases.
Drug Screening: In pharmaceutical research, Ac-LETD-AFC is employed in high-throughput screening assays to identify potential inhibitors or activators of caspase-8. Compounds that modulate caspase-8 activity can be identified by their effect on the fluorescence signal. This is valuable for the development of new therapeutic agents aimed at manipulating apoptosis for treating diseases such as cancer.
Toxicology Studies: Ac-LETD-AFC is also used in toxicology to evaluate the cytotoxic effects of chemicals and drug candidates on cells. By measuring caspase-8 activation, one can assess the extent of apoptosis induced by toxic substances. This aids in determining the potential safety and adverse effects of new pharmaceuticals or environmental toxins.
Basic Research: Beyond applied sciences, Ac-LETD-AFC serves as an important tool in basic research to study the intricacies of protease function and regulation. Researchers can use it to analyze the activity of purified caspase-8 and other related proteases in vitro. This contributes to a deeper understanding of protease biology and the development of assays for other proteolytic enzymes.
Recommended Services
Recommended Articles
- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
- Fluorescent Probes: Definition, Structure, Types and Application
- Fluorescent Dyes: Definition, Mechanism, Types and Application
- Coumarin Dyes: Definition, Structure, Benefits, Synthesis and Uses
- BODIPY Dyes: Definition, Structure, Synthesis and Uses
- Cyanine Dyes: Definition, Structure, Types and Uses
- Fluorescein Dyes: Definition, Structure, Synthesis and Uses
- Rhodamine Dyes: Definition, Structure, Uses, Excitation and Emission
- Unlocking the Power of Fluorescence Imaging: A Comprehensive Guide
- Cell Imaging: Definitions, Systems, Protocols, Dyes, and Applications
- Lipid Staining: Definition, Principles, Methods, Dyes, and Uses
- Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses
- Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses
- DNA Staining: Definition, Procedures, Benefits, Dyes and Uses
- Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
- Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
- Fluorescent Labeling: Definition, Principles, Types and Applications
Recommended Products
Online Inquiry
