5-carboxyfluorescein diacetate acetoxymethyl ester | 124412-00-6

Catalog Number	A18-0071
Category	Fluorescent Enzyme Substrates
Molecular Formula	C28H20O11
Molecular Weight	532.45

Catalog Number	Size	Price	Quantity
A18-0071	--	$--

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Product Introduction

5-carboxyfluorescein diacetate acetoxymethyl ester is a cell-permeant fluorogenic esterase substrate. It can be converted by nonspecific esterases of living cells from a membrane-permeable, nonpolar, nonfluorescent substance to a polar product CF, indicating the integrity of the plasma membrane.

Chemical Information
Application

Synonyms	5-CFDA-AM; acetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
Canonical SMILES	CC(=O)OCOC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C)OC5=C3C=CC(=C5)OC(=O)C)OC2=O
InChI	InChI=1S/C28H20O11/c1-14(29)34-13-35-26(32)17-4-7-21-20(10-17)27(33)39-28(21)22-8-5-18(36-15(2)30)11-24(22)38-25-12-19(37-16(3)31)6-9-23(25)28/h4-12H,13H2,1-3H3
InChI Key	YDBUJZYYYBVSEF-UHFFFAOYSA-N
Appearance	Solid Powder

5-Carboxyfluorescein diacetate acetoxymethyl ester (5-CFDA-AM), a fluorescent dye widely utilized in biological research, offers a myriad of applications. Here are four key applications of 5-CFDA-AM:

Cell Viability Assays: A cornerstone in cell viability assessment, 5-CFDA-AM is a go-to tool for evaluating cell viability and cytotoxicity across diverse cell types. This versatile dye infiltrates live cells and undergoes hydrolysis by intracellular esterases, yielding a fluorescent compound for precise quantification. This method furnishes a dependable gauge of living cell count within a culture, shedding light on cellular health and functionality.

Flow Cytometry: In the realm of flow cytometry, 5-CFDA-AM emerges as a pivotal agent for discerning between live and deceased cells. Its distinctive fluorescence is detectable and quantifiable as cells journey through a laser, furnishing critical insights into cell viability and population dynamics. This application stands paramount in assessing cellular well-being and responses in fields like immunology, cancer research, and beyond, enriching our comprehension of cellular behavior.

Tracking Cell Proliferation: Unlocking new vistas in cell proliferation tracking, 5-CFDA-AM facilitates the monitoring of cell replication by tracking the descent in fluorescence intensity across successive generations. As cells undergo division, the fluorescent signal wanes, enabling researchers to gauge the pace of cell proliferation. This methodology proves invaluable in scrutinizing cell cycle dynamics and the impact of growth factors on cellular growth, ushering in enhanced understanding.

Intracellular pH Measurement: Delving into the depths of intracellular processes, 5-CFDA-AM emerges as a stalwart tool for assessing intracellular pH in living cells. Upon entry into the cell, the dye transforms into a fluorescent variant sensitive to pH alterations, allowing for real-time monitoring of pH shifts. Through tracking these fluctuations, researchers delve into vital cellular processes such as metabolism, apoptosis, and signal transduction, where pH variations wield profound influence, expanding our comprehension of cellular dynamics.

Applications of Fluorescent Probes & Dyes