4,4-Difluoro-8(2'-aminophenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene | 929679-20-9

4,4-Difluoro-8(2'-aminophenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene is a fluorescent dye popularly used in scientific research due to its unique properties.

Fluorescence Microscopy: This compound is highly valued in fluorescence microscopy due to its intense and stable fluorescence emission. Researchers use it to label cellular components, enabling visualization of structures such as cell membranes, nuclei, and organelles. Its bright fluorescence facilitates high-resolution imaging, crucial for understanding cellular dynamics and structure-function relationships.

Flow Cytometry: In flow cytometry, this fluorescent dye is employed to tag and quantify various cell populations based on specific markers. By conjugating it with antibodies, researchers can simultaneously detect and analyze multiple cellular characteristics. This method is essential for applications like immunophenotyping and monitoring cell cycle stages in health and disease research.

Bioconjugation: The compound serves as an excellent choice for bioconjugation in studies aiming to track biomolecules. Its reactive amino group allows facile attachment to proteins, nucleic acids, and other biomolecules. This application is vital for creating fluorescent probes that can monitor biochemical processes in real-time, offering insights into molecular interactions and pathways.

Live-Cell Imaging: The photo-stability of this dye makes it ideal for live-cell imaging applications. By tagging live cells, researchers can observe cellular behaviors such as division, migration, and interaction with other cells in a dynamic environment. This is critical for understanding processes like embryonic development, cancer metastasis, and cellular responses to drugs.