1,5,7-Trimethyl-3-[6-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)ethenyl]-8-[4-(2,4-dinitrophenoxy)-phenyl]-4,4-difluoroboradiazasindacene | 1403241-80-4

1,5,7-Trimethyl-3-[6-(13-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)ethenyl]-8-[4-(2,4-dinitrophenoxy)-phenyl]-4,4-difluoroboradiazasindacene is a fluorophore compound with various applications in biological and chemical research.

Fluorescence Imaging: This compound is widely used as a fluorescent dye in imaging techniques, providing bright and stable fluorescence suitable for high-resolution microscopy. It can be used to label cells, proteins, and other biomolecules, enabling researchers to track and visualize biological processes in real time. Its unique spectral properties make it a favored choice for multicolor fluorescence imaging, enhancing the clarity and detail of observed structures.

Flow Cytometry: In flow cytometry, this compound is employed as a fluorescent marker to detect and sort cells based on specific characteristics. By conjugating it to antibodies or ligands, it allows the precise analysis of cell populations, such as immune cell subsets. This application is crucial for immunophenotyping and diagnostic purposes, offering insights into immune function and disease states.

Biosensing: The compound can be incorporated into biosensors to detect specific analytes or environmental changes. Its fluorescence changes in response to binding events or environmental shifts, providing a visible signal that can be quantitatively measured. This makes it an essential tool for developing sensors used in medical diagnostics and environmental monitoring.

Molecular Probes: As a component of molecular probes, this compound helps in studying intracellular environments, such as pH or ion concentrations. It can be designed to respond to specific cellular conditions, allowing researchers to observe how cells adapt to various stimuli. This is instrumental in advancing our understanding of cellular homeostasis and disease pathology.