1,5,7-Trimethyl-3-[3-amino-4-hydroxyphenyl)ethenyl]-8-phenyl-4,4-difluoroboradiazasindacene | 1802553-24-7

1,5,7-Trimethyl-3-[3-amino-4-hydroxyphenyl)ethenyl]-8-phenyl-4,4-difluoroboradiazasindacene, a fluorescent dye of significant importance in diverse scientific realms, finds extensive utility in the following key applications each:

Fluorescence Microscopy: Within the realm of fluorescence microscopy, this compound serves as a crucial fluorescent probe distinguished by its bright fluorescence and photostability. Its exceptional properties render it ideal for visualizing and analyzing cellular structures with remarkable resolution. Particularly valuable in immunofluorescence studies, it enables researchers to track the spatial distribution and expression patterns of proteins within cell structures.

Flow Cytometry: Essential in the domain of flow cytometry, a technique pivotal for analyzing the attributes of suspended cells or particles, this compound plays a pivotal role. By labeling cells with this fluorescent dye, researchers can categorize and segregate distinct cell populations based on their varying fluorescent intensities. This application holds profound significance in fields ranging from immunology to oncology, facilitating the in-depth study of cell populations and the evaluation of cellular functionalities.

Biomedical Imaging: In the arena of biomedical imaging, the fluorescent dye enhances the visualization of biological targets in intricate tissue environments. Employed in both in vitro and in vivo imaging methodologies, it amplifies the study of biological processes and disease progression. Through its robust fluorescence emission, researchers can capture detailed images conducive to the early detection and monitoring of various diseases.

Biochemical Assays: Within the realm of biochemical assays, this compound assumes a pivotal role in detecting and quantifying biomolecules. Through its conjugation to antibodies, substrates, or other biomolecules, it functions as a potent reporter molecule across diverse assays. Leveraging its fluorescent attributes, researchers can measure enzyme activities, binding interactions, and molecular concentrations, yielding invaluable insights in the realm of biochemical research and analysis.