1,3,5,7,8-Pentamethyl-2-bormo-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene | 1859909-59-3

1,3,5,7,8-Pentamethyl-2-bromo-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene is a fluorescent dye known for its unique photophysical properties.

Fluorescent Labeling: This compound is widely used as a fluorescent label in biological assays and imaging studies. Due to its high fluorescence quantum yield and stability, it provides bright and accurate signals in various imaging applications. Scientists use this dye to track cellular components and monitor biological processes in real-time, enhancing the understanding of intricate cellular dynamics.

Flow Cytometry: In flow cytometry, this fluorescent dye is employed to stain cells, enabling their detection and analysis based on fluorescence intensity. Its distinct spectral properties allow for multiplexing, where multiple markers can be assessed simultaneously. This is particularly useful in immunophenotyping, where different cell populations are identified and quantified in a mixed sample.

Fluorescent Probes in Chemistry: In chemistry, this compound acts as a fluorescent probe to study molecular interactions and dynamics in various solvents and conditions. Researchers utilize it to detect and quantify the presence of analytes or to investigate solvent polarity effects. Its sensitivity to environment changes makes it a powerful tool in chemical sensing applications.

Live Cell Imaging: This dye is beneficial in live cell imaging to visualize subcellular structures and dynamic processes without compromising cell viability. Its low photobleaching and high signal-to-noise ratio ensure prolonged observation of cellular events. This application is crucial for studies involving cell motility, division, and structural organization.