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Rhod-5N AM
| Catalog Number | A14-0025 |
| Category | Calcium, Chloride and Other indicators |
| Molecular Formula | C51H56BrN5O21 |
| Molecular Weight | 1154.9 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0025 | -- | -- |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Rhod-5N AM is a cell-permeant acetoxymethyl ester of rhod-5N, a fluorescent calcium indicator. Rhod-5N AM is hydrolyzed by intracellular esterases to release rhod-5N in cells. Rhod-5N AM is useful for studying compartments with high concentrations of calcium for its low affinity.
Chemical Information
Product Specification
Application
| Synonyms | Rhod-5N Acetoxymethyl ester; N-(9-(4-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)-3-(2-(2-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)-5-nitrophenoxy)ethoxy)phenyl)-6-(dimethylamino)-3H-xanthen-3-ylidene)-N-methylmethanaminium bromide |
| Purity | ≥90% |
| Canonical SMILES | CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=C(C=C(C=C1)[N+](=O)[O-])OCCOC2=CC(=C3C4=C(C=C(C=C4)N(C)C)OC5=C3C=CC(=C5)N(C)C)C=CC2=[N+](CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C.[Br-] |
| InChI | InChI=1S/C51H56N5O21.BrH/c1-31(57)69-27-73-47(61)23-54(24-48(62)74-28-70-32(2)58)41-15-9-35(51-39-13-10-36(52(5)6)20-43(39)77-44-21-37(53(7)8)11-14-40(44)51)19-45(41)67-17-18-68-46-22-38(56(65)66)12-16-42(46)55(25-49(63)75-29-71-33(3)59)26-50(64)76-30-72-34(4)60;/h9-16,19-22H,17-18,23-30H2,1-8H3;1H/q+1;/p-1 |
| InChIKey | PMQMSJPQRCLPMW-UHFFFAOYSA-M |
| Appearance | Solid Powder |
| Excitation | 551 nm |
| Emission | 576 nm |
| Storage | Store at -20°C |
Rhod-5N AM is a cell-permeant acetoxymethyl ester derivative of rhod-5N, which is a highly effective fluorescent calcium indicator known for its ability to permeate cell membranes. Once inside the cell, Rhod-5N AM is hydrolyzed by intracellular esterases, releasing the active form, rhod-5N. This activation allows Rhod-5N to bind to calcium ions within the cell, enabling researchers to study calcium dynamics in various cellular compartments. Rhod-5N AM is particularly valuable for its low affinity for calcium ions, making it ideal for tracking large fluctuations and concentrations of calcium, especially in environments where calcium levels are significantly elevated.
One key application of Rhod-5N AM is in neuroscience, where it is used to study synaptic activity and neuronal communication. Calcium ions play a crucial role in neurotransmitter release at synapses, and monitoring these ions can provide insights into the fundamental mechanisms of brain function and plasticity. By using Rhod-5N AM, scientists can visualize calcium transients and gain a deeper understanding of neuronal signaling pathways and the effects of various neurochemical modulators.
Another important application is in cardiology research. Rhod-5N AM is employed to study cardiac muscle contractions, as calcium ions are central to the contraction mechanism in heart cells. By tracking calcium waves in cardiac tissues, researchers can investigate conditions like arrhythmias and other cardiac dysfunctions. This understanding can lead to the development of new therapeutic strategies and interventions for heart disease.
Moreover, Rhod-5N AM is used in cell biology to examine calcium dynamics in organelles such as mitochondria and endoplasmic reticulum, where calcium plays a critical role in metabolic regulation and signaling. By providing insights into calcium homeostasis and fluctuations within these cellular compartments, Rhod-5N AM facilitates research into cellular metabolism, apoptosis, and other cell physiology aspects.
Lastly, Rhod-5N AM is applied in pharmacological research to assess the impact of drugs on calcium signaling pathways. Since many drugs exert their effects through modulation of calcium channels and pumps, using Rhod-5N AM allows researchers to evaluate drug efficacy and toxicity by visualizing changes in intracellular calcium levels. This application is crucial for the drug development process and for ensuring the safety and effectiveness of new pharmaceutical compounds.
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