Golgi Fluorescent Probe

The Golgi apparatus is an organelle composed of a number of flat vesicles with secretion as a primary function. The Golgi is a highly polar organelle formed by the stacking of several flat vesicles. It is often distributed between the endoplasmic reticulum and the cell membrane, and is arcuate or hemispherical. The convex one facing the endoplasmic reticulum is called a forming face or a cis face. The concave one facing the plasma membrane is called a mature face or a trans face. There are some large or small transport vesicles on both the cis and the back. In polar cells, the Golgi is often distributed in a large amount in the cytoplasm at the secretory end. Because it looks very like the smooth endoplasmic reticulum, some scientists believe that it evolved from the smooth endoplasmic reticulum.

Figure 1. Structure of Golgi apparatus.

Functions

The Golgi apparatus is the primary collection and delivery station for protein products received from the endoplasmic reticulum (ER). The protein synthesized in the ER is packaged into vesicles and then fused to the Golgi apparatus. These cargo proteins are modified to be secreted by exocytosis or used in cells. The main function of the Golgi apparatus is to process, sort, and transport proteins synthesized by the endoplasmic reticulum, which are then sent to specific parts of the cell or secreted outside the cell. The Golgi is the site where the final processing and packaging of cell secretions (such as proteins) is completed. The vesicles from the endoplasmic reticulum fuse with the Golgi membrane and deliver the contents into the Golgi cavity where the newly synthesized protein peptide chain continues to be modified and packaged. The Golgi also synthesizes some polysaccharides secreted into the extracellular and modified cell membranes.

Golgi Fluorescent Probe

The Golgi is an organelle found in most eukaryotic cells in which proteins, lipids and carbohydrates are prepared for secretion from cells or by other organelles within the cell. The Golgi contains a group of glycosylation enzymes that attach various sugar monomers to the protein as it moves through the device. BOC Sciences provides a cell-permeable probe that selectively stains Golgi complexes for lipid metabolism and transport studies can be used for live cell Golgi-specific fluorescence staining. The Golgi is primarily responsible for the correct classification of lipids and proteins in cells. Therefore, most of the cell-permeable probes of these organelles are lipids or chemicals that affect protein movement. The most effective probes for Golgi are fluorescent ceramides and sphingolipids. Golgi markers are commonly used to identify and delineate their respective target organelles in live cell imaging studies of protein trafficking.

Reference:

Fabene PF.; et al. 1898-1998: Camillo Golgi and "the Golgi": one hundred years of terminological clones. Brain Research Bulletin. 1998, 47 (3): 195–8.