
Sulfo-Cyanine5 maleimide | 2242791-82-6
Catalog Number | F03-0008 |
Category | sulfo-Cyanine5 |
Molecular Formula | C38H43KN4O9S2 |
Molecular Weight | 803.00 |
Catalog Number | Size | Price | Quantity |
---|---|---|---|
F03-0008 | -- | $-- |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.An analog without sulfo groups, Cyanine5 maleimide, is also available.
- Chemical Information
- Product Specification
- Application
Related CAS | 2130955-10-9 (inner salt) 2242791-81-5 (inner salt) 2301999-22-2 (sodium salt) |
Purity | NMR 1H, HPLC-MS (95+%) |
Solubility | soluble in water, DMSO, DMF |
Appearance | dark blue powder |
ε, L⋅mol-1⋅cm-1 | 271000 |
Fluorescence Quantum Yield | 0.28 |
Excitation | 646 |
Emission | 662 |
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Sulfo-Cyanine5 maleimide, a versatile fluorescent dye, finds widespread use in biological research owing to its vibrant fluorescence and reactive maleimide group. Here are four key applications of Sulfo-Cyanine5 maleimide:
Fluorescence Labeling: Widely employed for labeling proteins, peptides, and other biomolecules, Sulfo-Cyanine5 maleimide interacts specifically with thiol groups on cysteine residues, ensuring stable and precise conjugation. This capability is particularly advantageous in live-cell imaging, flow cytometry, and fluorescence microscopy, providing a powerful tool to monitor and visualize molecular interactions in diverse biological contexts.
Protein-Protein Interaction Studies: Leveraging Sulfo-Cyanine5 maleimide, researchers delve into the intricate world of protein-protein interactions through advanced techniques like Förster Resonance Energy Transfer (FRET). By labeling interacting proteins with distinct fluorophores, changes in fluorescence intensity can be scrutinized, offering insights into binding events and interaction dynamics. This approach sheds light on the intricate molecular mechanisms governing complex biological processes.
Quantitative Western Blotting: In the realm of quantitative Western blotting, Sulfo-Cyanine5 maleimide plays a pivotal role in providing accurate and sensitive protein detection. Following protein labeling on the blot, fluorescent signals are quantified using sophisticated imaging systems, enabling precise measurement of protein levels. This precision facilitates in-depth investigations into protein expression, degradation pathways, and post-translational modifications, advancing our understanding of cellular processes.
In Vivo Imaging: Sulfo-Cyanine5 maleimide emerges as a crucial tool in in vivo imaging studies, enabling real-time tracking of biomolecules and cells within living organisms. Its near-infrared fluorescence properties minimize background autofluorescence, facilitating deep tissue penetration for enhanced imaging depth. Particularly valuable for monitoring cellular trafficking, tumor imaging, and studying dynamic biological processes within animal models, this application showcases the versatility and utility of Sulfo-Cyanine5 maleimide in cutting-edge research endeavors.
Recommended Services
Blogs & Technical Articles

- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
- Fluorescent Probes: Definition, Structure, Types and Application
- Fluorescent Dyes: Definition, Mechanism, Types and Application
- Coumarin Dyes: Definition, Structure, Benefits, Synthesis and Uses
- BODIPY Dyes: Definition, Structure, Synthesis and Uses
- Cyanine Dyes: Definition, Structure, Types and Uses
- Fluorescein Dyes: Definition, Structure, Synthesis and Uses
- Rhodamine Dyes: Definition, Structure, Uses, Excitation and Emission
- Unlocking the Power of Fluorescence Imaging: A Comprehensive Guide
- Cell Imaging: Definitions, Systems, Protocols, Dyes, and Applications
- Lipid Staining: Definition, Principles, Methods, Dyes, and Uses
- Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses
- Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses
- DNA Staining: Definition, Procedures, Benefits, Dyes and Uses
- Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
- Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
- Fluorescent Labeling: Definition, Principles, Types and Applications
Recommended Products