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JOE alkyne, 6-isomer
| Catalog Number | R02-0028 |
| Category | JOE Dyes |
| Molecular Formula | C26H17NCl2O8 |
| Molecular Weight | 542.32 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
JOE dye is a fluorophore similar to TET, but less hydrophobic. The dye is often used for the labeling of nucleic acids.JOE alkyne can be conjugated with azides using copper catalyzed Click chemistry reaction.
Chemical Information
Product Specification
Application
Computed Properties
| Purity | NMR 1H, HPLC-MS (95%) |
| IUPAC Name | 4',5'-dichloro-3',6'-dihydroxy-2',7'-dimethoxy-1-oxo-N-prop-2-ynylspiro[2-benzofuran-3,9'-xanthene]-5-carboxamide |
| Canonical SMILES | COC1=C(C(=C2C(=C1)C3(C4=CC(=C(C(=C4O2)Cl)O)OC)C5=C(C=CC(=C5)C(=O)NCC#C)C(=O)O3)Cl)O |
| InChI | InChI=1S/C26H17Cl2NO8/c1-4-7-29-24(32)11-5-6-12-13(8-11)26(37-25(12)33)14-9-16(34-2)20(30)18(27)22(14)36-23-15(26)10-17(35-3)21(31)19(23)28/h1,5-6,8-10,30-31H,7H2,2-3H3,(H,29,32) |
| InChIKey | FITDEERKHNKYNG-UHFFFAOYSA-N |
| Solubility | good in DMF, DMSO |
| Appearance | orange solid |
| ε, L⋅mol-1⋅cm-1 | 75000 |
| Excitation | 533 |
| Emission | 554 |
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
JOE alkyne 6-isomer, a versatile fluorescent dye, finds extensive application in molecular and cellular biology. Here are four key applications:
Fluorescent Labeling: JOE alkyne 6-isomer serves as a pivotal tool for labeling biomolecules such as nucleic acids and proteins through click chemistry reactions. This specialized fluorescent labeling technique enables researchers to visualize and track specific molecules within intricate biological systems. By leveraging this method, scientists delve into cellular processes like DNA replication, transcription, and protein-protein interactions in real-time, unraveling the dynamic intricacies of biomolecular interactions.
Flow Cytometry: In the realm of flow cytometry, JOE alkyne 6-isomer plays a critical role in staining cells for sorting and analysis. Through conjugation of this dye to antibodies or other targeting molecules, distinct cell populations can be discerned and quantified based on their unique fluorescence signals. This application is integral for immunophenotyping, cell cycle analysis, and exploring cellular responses to diverse treatments, shedding light on the cellular landscape with precision and clarity.
Fluorescence Microscopy: Renowned for its exceptional performance in fluorescence microscopy, JOE alkyne 6-isomer enables high-resolution imaging of cellular structures and dynamics. Its vibrant fluorescence and robust stability render it ideal for both live-cell imaging and fixed samples, offering insights into intricate cellular architecture, intracellular trafficking, and the spatial arrangement of biomolecules.
Quantitative PCR (qPCR): A key player in quantitative PCR assays, JOE alkyne 6-isomer acts as a fluorescent reporter molecule, facilitating the precise quantification of nucleic acids. By monitoring the fluorescence signal during the amplification process, researchers can delve into gene expression analysis, pathogen detection, and genetic variation studies with unparalleled accuracy.
| XLogP3 | 4 |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 8 |
| Rotatable Bond Count | 4 |
| Exact Mass | 541.0331219 g/mol |
| Monoisotopic Mass | 541.0331219 g/mol |
| Topological Polar Surface Area | 124Ų |
| Heavy Atom Count | 37 |
| Formal Charge | 0 |
| Complexity | 920 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
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