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Cyanine3 maleimide
| Catalog Number | F02-0004 |
| Category | Cyanine3 |
| Molecular Formula | C36H43N4O3BF4 |
| Molecular Weight | 666.56 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.Labeling with Cyanine3 maleimide is selective, and efficient.
Chemical Information
Product Specification
Application
| Related CAS | 1593644-29-1 (without anion) |
| Purity | NMR 1H, HPLC-MS (95%) |
| Solubility | well soluble in DMSO (0.50 M = 330 g/L), in DMF, dichloromethane, very poorly soluble in water (0.57 mM = 420 mg/L) |
| Appearance | red powder |
| ε, L⋅mol-1⋅cm-1 | 150000 |
| Fluorescence Quantum Yield | 0.31 |
| Excitation | 555 |
| Emission | 570 |
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Cyanine3 maleimide, a fluorescent dye prevalent in various biological and biochemical applications, exhibits a plethora of uses in modern research. Here are four key applications of Cyanine3 maleimide:
Fluorescence Microscopy: A staple in the realm of fluorescence microscopy, Cyanine3 maleimide serves as a beacon for labeling proteins and nucleic acids within cellular domains. Its radiant fluorescence aids in the visualization of intricate cellular structures, the tracking of protein whereabouts, and the real-time scrutiny of cellular processes. Researchers lean on its unwavering stability and pinpoint specificity to achieve high-resolution imaging, unveiling a microscopic universe teeming with vibrant detail.
Flow Cytometry: Within the intricate landscape of flow cytometry, Cyanine3 maleimide emerges as a pivotal player in the labeling of antibodies and ligands for the precise detection and quantification of distinct cell populations. The dye’s distinct fluorescent signature facilitates multiparametric analysis, effectively delineating between a myriad of fluorophores. This application stands as a cornerstone for immunophenotyping and other intricate cellular analyses demanding a nuanced understanding of cellular dynamics.
Protein Labeling: Embarking on a journey into the microcosm of protein labeling, Cyanine3 maleimide unveils its specificity in reacting with thiol groups, rendering it a prime candidate for labeling cysteine residues within proteins. This strategic maneuver empowers researchers to delve into the complexities of protein-protein interactions, protein folding dynamics, and conformational metamorphoses. The labeled proteins emerge as beacons in the vast sea of cellular processes, easily discernible through an array of fluorescence-based assays, shedding light on the hidden intricacies of protein behavior.
Molecular Probes: Navigating the terrain of molecular probes, Cyanine3 maleimide assumes a critical role in crafting diagnostic and research tools of unparalleled sensitivity. By conjugating the dye with biomolecules like antibodies, peptides, or oligonucleotides, researchers can engineer highly sensitive assays tailored for the detection of specific targets. These probes serve as invaluable assets in a myriad of tasks, from pathogen detection to disease marker monitoring and the orchestration of molecular imaging studies, unlocking a realm of molecular insights previously obscured from view.
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