BODIPY 576/589 SE | CAS 201998-61-0

BODIPY 576/589 SE is a BODIPY-based fluorescent dye supplied as an N-hydroxysuccinimide (SE) ester, enabling efficient covalent labeling of primary amines in proteins, peptides, and other amine-containing biomolecules. With excitation in the visible range and emission centered around the 580-590 nm region, it is frequently used for fluorescence labeling workflows where bright, photostable signal is required for microscopy and quantitative fluorescence readouts.

1. Protein Amine Labeling

BODIPY 576/589 SE is routinely used by protein chemistry and chemical biology groups to generate fluorescently labeled proteins for downstream imaging and assay development. Because the SE ester reacts with primary amines, researchers commonly label lysine residues and N-termini to produce conjugates for fluorescence microscopy, plate-based fluorescence measurements, and binding studies where a stable covalent fluorophore attachment is preferred. This labeling format is also widely used when fluorescent conjugates must be prepared reproducibly for comparative experiments across multiple protein batches.

2. Antibody And Peptide Conjugates

BODIPY 576/589 SE supports fluorescent labeling of antibodies and peptides for immunofluorescence and targeted imaging reagent development in cell biology laboratories. The NHS ester chemistry enables straightforward preparation of amine-reactive antibody conjugates used to visualize antigen distribution or to track peptide localization in cellular assays. In fluorescence workflows that rely on visible-channel detection, the dye's emission near 580-590 nm helps align labeled biomolecules with common filter sets used for green-to-orange fluorescence imaging and quantitative signal collection.

3. Fluorescent Probe Construction

BODIPY 576/589 SE is frequently incorporated into fluorescent probe and tracer construction where a covalent fluorophore handle is needed for conjugate assembly. Researchers use the dye to prepare labeled biomolecule building blocks (for example, amine-functional peptides or carrier proteins) that are later integrated into larger imaging reagents, affinity-based probes, or multicomponent fluorescence constructs. This approach is particularly useful when the fluorophore must remain attached under washing, dilution, or extended incubation conditions typical of microscopy and plate-reader assays.

4. Flow Cytometry Labeling

BODIPY 576/589 SE is used to prepare fluorescently labeled biomolecules for flow cytometry workflows that require amine-targeted covalent tagging. Laboratories often label proteins, antibodies, or other amine-containing reagents to monitor binding to cell-associated targets or to quantify fluorescent signals in suspension. The dye's emission in the 580-590 nm region supports detection in appropriate fluorescence channels, enabling researchers to track labeled reagent association and compare relative fluorescence intensities across experimental conditions.