
BODIPY 493/503 NHS Ester | 216961-98-7
Catalog Number | F01-0166 |
Category | BODIPY |
Molecular Formula | C20H22BF2N3O4 |
Molecular Weight | 417.22 |
Catalog Number | Size | Price | Quantity |
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F01-0166 | 100 mg | $1698 | |
F01-0166 | 1 g | $8298 |
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Product Introduction
BODIPY 493/503 NHS Ester is a green-fluorescent dye that is reactive with primary amines on peptides, proteins, modified nucleotides, biopolymers, and other amine-containing biomolecules. This compound is relatively insensitive to environment and pH changes.
Chemical Information |
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Synonyms | 10-(3-((2,5-Dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)-5,5-difluoro-1,3,7,9-tetramethyl-5H-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-4-ium-5-uide |
Appearance | Solid Powder |
- Application
BODIPY 493/503 NHS Ester is a fluorescent dye commonly used for labeling and detection in various biological applications. Here are some key applications of BODIPY 493/503 NHS Ester:
Fluorescence Microscopy: BODIPY 493/503 NHS Ester is widely used in fluorescence microscopy for its bright and stable emission. It can be conjugated to biomolecules such as proteins or antibodies, enabling the visualization of specific cellular components. This assists researchers in studying cellular structures and dynamics with high specificity and resolution.
Flow Cytometry: In flow cytometry, BODIPY 493/503 NHS Ester is used to label cells, allowing for the analysis of cell populations based on fluorescence intensity. By tagging surface or intracellular markers, scientists can differentiate cell types, assess cell health, and evaluate functional responses. Its strong fluorescence and compatibility with other dyes make it ideal for multiparametric analyses.
Protein Labeling: BODIPY 493/503 NHS Ester is an excellent choice for labeling proteins due to its efficient conjugation via the NHS ester group. This facilitates the study of protein-protein interactions, localization, and cellular trafficking. The dye’s spectral properties allow for multiplexing with other labels, enhancing the depth of protein research.
Lipid Research: The lipophilic nature of BODIPY 493/503 NHS Ester makes it suitable for labeling lipids and studying lipid metabolism. By integrating into lipid structures, it enables visualization of lipid droplets and membranes in living cells. This helps researchers investigate lipid storage dynamics and their role in health and disease.
Applications of Fluorescent Probes & Dyes
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