Nitric Oxide Probes

NO is a gas at room temperature, and its fat solubility is one of the possible factors that make it a messenger molecule in the human body. It does not require any intermediary mechanism to rapidly diffuse through the biofilm, transmitting information generated by one cell to the cells around it, the main influencing factor is its biological half-life. It has many biological functions, which are characterized by free radicals, which are easy to participate in and transfer electron reactions and are added to the body's redox process. The coordination of the molecule makes it highly compatible with heme iron and non-heme iron to replace the positions of O₂ and CO₂. According to research reports, hemoglobin-NO can lose its nearby base and become free of heme-NO, which means that free bases can freely participate in the catalytic reaction, free proteins can freely change conformation, free The heme can be freely diffused from the protein, and any one of these three changes or a combination thereof will play an important role in the activation of guanylate cyclase . The biological role of NO and its mechanism of action are in the ascendant, and its findings suggest the prospect of inorganic molecules in the medical field. Nitric oxide is the smallest, lightest, and simplest information-transmitting molecule in the body that has been discovered so far, and plays an important role in life. Since the concentration of nitric oxide is closely related to the physiological function of the body, and its half-life is very short, it brings certain difficulties to its rapid detection, thus establishing high sensitivity, high selectivity tissue and intracellular nitric oxide concentration and The analysis method of space-time distribution is of great significance.

Nitric Oxide Probes

Biological function

Nitric oxide, known as endothelium-derived relaxing factor (EDRF), is endogenously synthesized from L-arginine, oxygen and NADPH by various nitric oxide synthase (NOS) enzymes. Reduction of inorganic nitrates can also be used to make nitric oxide. One of the major enzymatic targets of nitric oxide is guanylate cyclase. In the presence of iron, nitric oxide binds to the heme region of the enzyme leading to activation. Nitric oxide is highly reactive (life is a few seconds) but can diffuse freely on the membrane. These properties make nitric oxide ideal for transient paracrine (between adjacent cells) and autocrine (single intracellular) signaling molecules. Once nitric oxide is converted to nitrate and nitrite by oxygen and water, the cellular signal is inactivated.

Nitric Oxide Probes

The nitric oxide probe provided by BOC Sciences, the complex itself has no fluorescence, and when reacted with NO, it produces fluorescence under excitation light, and the fluorescence intensity increases with increasing NO concentration, and exists in hydrogen peroxide and nitrate. In the case of fluorescent probes showing higher selectivity, our nitric oxide probes enable direct detection of NO.

Features

NO produced by tissue and cultured cells can be observed in real time.

Visible light excitation minimizes the effects of fluorescence from biological components.

Visible light excitation makes it difficult to damage cells.

Since it captures NO under neutral conditions and fluoresces, it can be measured without changing the pH.

Highly sensitive and specific measurements are possible.

Reference:

Perez, Krystle M.; et al. Sildenafil in Term and Premature Infants: A Systematic Review. Clinical Therapeutics. 2015, 37 (11): 2598–2607.e1.

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