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BODIPY 493/503 | CAS 121207-31-6
| Catalog Number | R12-0001 |
| Category | BODIPY |
| Molecular Formula | C14H17BF2N2 |
| Molecular Weight | 262.1 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| R12-0001 | -- | -- |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BODIPY 493/503 is a fluorescent probe for labeling cellular neutral lipid contents in live and fixed cells. BODIPY 493/503 has been used in epifluorescent, confocal, two-photon microscopy, and flow cytometry.
Chemical Information
Product Specification
Application
| Synonyms | BDP 493/503 lipid stain;Pyrromethene 546; 1,3,5,7,8-Pentamethyl-BODIPY; (T-4)-[2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)ethyl]-3,5-dimethyl-1H-pyrrolato-κN]difluoro-boron |
| Purity | ≥98% |
| Canonical SMILES | [B-]1(N2C(=CC(=C2C(=C3[N+]1=C(C=C3C)C)C)C)C)(F)F |
| InChI | InChI=1S/C14H17BF2N2/c1-8-6-10(3)18-13(8)12(5)14-9(2)7-11(4)19(14)15(18,16)17/h6-7H,1-5H3 |
| InChIKey | DRJHPEGNOPSARR-UHFFFAOYSA-N |
| Solubility | good in many organic solvents |
| Appearance | Red Solid |
| Excitation | 493 nm |
| Emission | 503 nm |
| Storage | Store at -20°C |
BODIPY 493/503, a fluorescent dye renowned for its exceptional photostability and vibrant emission, finds widespread usage in diverse biological realms. Here are four key applications of BODIPY 493/503:
Fluorescence Microscopy: At the forefront of fluorescence microscopy, BODIPY 493/503 is a staple choice for labeling and visualizing cellular components. When linked to biomolecules like proteins or lipids, it enables researchers to scrutinize cellular structures and dynamics with unparalleled precision. Its adaptability to live cell imaging renders it a prime candidate for real-time investigations into cell behavior.
Flow Cytometry: In the realm of flow cytometry, BODIPY 493/503 emerges as a fluorescent beacon for identifying and quantifying distinct cell populations based on specific markers. Leveraging this dye, scientists probe various cellular attributes such as size, granularity, and protein expression levels. This application plays a pivotal role in immunophenotyping, cell sorting, and clinical diagnostics.
Lipophilic Staining: Renowned for its high lipophilicity, BODIPY 493/503 stands out as a potent tool for staining lipid droplets and other hydrophobic structures within cells. Researchers harness this distinctive property to delve into lipid metabolism, storage, and distribution across different cell types. This staining method proves invaluable in unraveling diseases linked to lipid metabolism, such as obesity and fatty liver disease.
Protein Labeling: Enabling a myriad of biochemical assays, BODIPY 493/503 can be conjugated to proteins, antibodies, and diverse biomolecules. By attaching proteins with this fluorescent dye, researchers dive into protein interactions, localization, and dynamics, shedding light on intricate cellular processes. This comprehensive exploration of protein functions through in vitro and in vivo studies enhances our comprehension of cellular dynamics and mechanisms.
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