4-Methylumbelliferyl 2-sulfamino-2-deoxy-α-D-Glucopyranoside sodium salt | 460085-45-4

Catalog Number	A18-0105
Category	Fluorescent Enzyme Substrates
Molecular Formula	C16H18NNaO10S
Molecular Weight	439.37

Catalog Number	Size	Price	Quantity
A18-0105	--	$--

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Product Introduction

4-Methylumbelliferyl 2-sulfamino-2-deoxy-α-D-Glucopyranoside (4-MU-α-GlcNS) is a fluorogenic substrate for heparin sulphamidase. 4-MU-α-GlcNS is cleaved by heparin sulphamidase to yield 4-MU-α-GlcNH2, which is then cleaved by α-glucosaminidase to release the fluorescent moiety 4-MU. 4-MU-α-GlcNS has been used to quantify heparin sulphamidase deficiencies associated with Mucopolisaccaridosis IIIA and other lysosomal disorders.

Chemical Information
Related CAS	180088-52-2 (free acid)
Synonyms	4-MU-α-GlcNS sodium salt; 2H-1-Benzopyran-2-one, 7-[[2-deoxy-2-(sulfoamino)-α-D-glucopyranosyl]oxy]-4-methyl-, sodium salt (1:1); 7-[[2-deoxy-2-(sulfoamino)-α-D-glucopyranosyl]oxy]-4-methyl-2H-1-benzopyran-2-one monosodium salt
Purity	≥98%
IUPAC Name	sodium;N-[(2R,3R,4R,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-2-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]sulfamate
Canonical SMILES	CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)CO)O)O)NS(=O)(=O)[O-].[Na+]
InChI	InChI=1S/C16H19NO10S.Na/c1-7-4-12(19)26-10-5-8(2-3-9(7)10)25-16-13(17-28(22,23)24)15(21)14(20)11(6-18)27-16;/h2-5,11,13-18,20-21H,6H2,1H3,(H,22,23,24);/q;+1/p-1/t11-,13-,14-,15-,16+;/m1./s1
InChI Key	LMECUNAMBHBGFU-MYDYNYEJSA-M
Appearance	White Solid
Melting Point	224-229°C (dec.)

Product Specification
Application

Storage

Store at -20°C

4-Methylumbelliferyl 2-sulfamino-2-deoxy-α-D-glucopyranoside sodium salt, a fluorogenic substrate utilized in diverse biochemical assays, possesses a multitude of valuable applications. Here are four key applications:

Enzyme Activity Assays: Widely employed to assess the activity of specific enzymes like N-acetylglucosaminidases, this compound triggers the release of 4-methylumbelliferone, a fluorescent byproduct, upon enzymatic cleavage. The quantifiable nature of this fluorescence enables researchers to track and measure enzyme kinetics in real-time with exceptional sensitivity.

Disease Diagnosis: In clinical practice, this substrate emerges as a crucial tool for identifying enzyme deficiencies linked to lysosomal storage disorders such as Sanfilippo syndrome. By gauging enzymatic activity levels in patient samples, it facilitates early disease detection and monitoring, aiding in precise clinical evaluations and tailored treatment strategies.

High-Throughput Screening: Leveraging its distinctive fluorogenic attributes, 4-methylumbelliferyl 2-sulfamino-2-deoxy-α-D-glucopyranoside sodium salt stands out as an optimal choice for high-throughput screening endeavors in drug discovery. The rapid generation of fluorescent signals post-enzymatic cleavage allows for swift, automated assessment of enzyme activity across extensive sample collections, expediting the discovery of potential therapeutic agents.

Biochemical Research: A cornerstone in biochemical and molecular biology studies, this compound serves as a vital instrument for exploring enzyme mechanisms and substrate specificity. Researchers deploy it to delve into the catalytic characteristics of enzymes and craft inhibitors or activators. This deepens our comprehension of enzyme functionality, paving the way for the development of enzyme-targeted therapeutic interventions.

Applications of Fluorescent Probes & Dyes