5-Carboxyfluorescein N-succinimidyl ester | 92557-80-7

5-Carboxyfluorescein N-succinimidyl ester, a versatile fluorescent dye, finds wide application in diverse scientific and medical fields. Here are four key applications of this compound:

Cell Labeling: This fluorescent dye, 5-Carboxyfluorescein N-succinimidyl ester, serves as a valuable tool for tagging cells to track their movements and distributions in complex biological systems. By conjugating this fluorescent marker to cellular constituents, researchers can observe the real-time behaviors of cells, including migration, proliferation, and differentiation. This application proves particularly significant in studies examining immune cell trafficking and the metastatic spread of cancer.

Flow Cytometry: A critical component of flow cytometry, 5-Carboxyfluorescein excels in probing the physical and chemical attributes of cells or particles. When cells are stained with this dye, their fluorescence allows for easy identification and quantification, facilitating the precise sorting and analysis of heterogeneous cell populations. This technique enables researchers to delve into cell populations with unparalleled accuracy.

Protein Labeling: 5-Carboxyfluorescein N-succinimidyl ester emerges as a powerful tool for tagging proteins in a myriad of biochemical assays. By covalently linking the dye to proteins, scientists can scrutinize protein interactions, subcellular localization, and functional roles within cellular contexts. This method proves indispensable for unraveling protein dynamics and finds applications in techniques like ELISA, elevating our understanding of protein behavior.

Live Cell Imaging: Through its extensive use in live cell imaging, 5-Carboxyfluorescein N-succinimidyl ester enables researchers to visualize dynamic cellular processes within living organisms. By integrating this fluorescent compound into live cells, scientists can monitor a plethora of dynamic events, such as intracellular transport, signal transduction, and organelle activities.