DBCO-PEG-Silane, MW 5K

DBCO-PEG-Silane MW 5K is a multifunctional reagent used extensively in bioconjugation and surface modification applications. Here are some key applications of DBCO-PEG-Silane MW 5K:

Surface Functionalization: DBCO-PEG-Silane MW 5K is commonly used for the functionalization of glass and silica surfaces in biosensor development. By attaching to surfaces via its silane group and presenting a DBCO group for subsequent cycloaddition reactions, it provides a versatile platform for subsequent biomolecular immobilization. This results in highly functionalized surfaces with minimal non-specific binding, essential for sensitive detection in diagnostic assays.

Nanoparticle Modification: This reagent is ideal for modifying nanoparticles with biological or chemical functionalities. The PEG spacer in DBCO-PEG-Silane helps to improve the colloidal stability of nanoparticles in biological environments, thereby reducing aggregation and non-specific interactions. The DBCO group enables bio-orthogonal conjugation with azide-functionalized molecules, allowing for targeted delivery or enhanced imaging capabilities.

Hydrogel Fabrication: DBCO-PEG-Silane is employed in the fabrication of hydrogels for tissue engineering and drug delivery systems. By incorporating this bifunctional reagent, hydrogels can be designed with specific mechanical properties and functionalities. This allows for controlled release of therapeutics or the creation of scaffolds that can mimic the extracellular matrix in cell culture applications.

Microarray Development: The use of DBCO-PEG-Silane in microarray fabrication enhances the immobilization of biomolecules on glass slides. This reagent provides a stable attachment point for DNA, RNA, or protein capture probes through bio-orthogonal click chemistry. As a result, microarrays produced with DBCO-PEG-Silane show improved sensitivity and specificity, making them more effective for high-throughput screening and diagnostic applications.