Anti-Rabbit IgG-ATTO 594 antibody produced in goat

Anti-Rabbit IgG-ATTO 594 antibody produced in goat is a specialized fluorescent antibody used widely in immunological and cell biology studies. Here are some key applications of Anti-Rabbit IgG-ATTO 594 antibody produced in goat:

Immunofluorescence Microscopy: This antibody is used to visualize specific target proteins in cells and tissues by binding to rabbit primary antibodies. The ATTO 594 fluorophore emits red fluorescence, allowing researchers to clearly see protein localization and distribution under a fluorescence microscope. It is particularly useful for multi-color staining experiments where different proteins need to be distinguished simultaneously.

Flow Cytometry: In flow cytometry, Anti-Rabbit IgG-ATTO 594 is employed to detect and quantify rabbit primary antibody-bound antigens on the surface or inside cells. The bright fluorescence of ATTO 594 facilitates the precise identification and sorting of cell populations based on the expression of specific markers. This application is essential for immunophenotyping, cell cycle analysis, and other cellular assays.

Western Blotting: This antibody can be used in Western blotting to detect rabbit primary antibodies bound to specific proteins on a membrane. After transferring proteins from SDS-PAGE gels to membranes, the Anti-Rabbit IgG-ATTO 594 antibody binds to the primary antibody, and its fluorescence is then detected using appropriate imaging systems. This enables accurate visualization and quantification of target proteins in complex mixtures.

Live Cell Imaging: Anti-Rabbit IgG-ATTO 594 can also be adapted for live cell imaging applications where real-time monitoring of protein interactions and dynamics is required. By using this antibody in conjunction with rabbit primary antibodies that recognize live cell surface markers, researchers can track these proteins in living cells without disrupting cellular functions. This application is valuable for studying dynamic biological processes such as protein trafficking, signaling, and cellular responses.