6-Carboxyfluorescein N-succinimidyl ester | 92557-81-8

6-Carboxyfluorescein N-succinimidyl ester (6-FAM SE) is a fluorescent dye commonly utilized for labeling biomolecules. Here are four key applications of 6-Carboxyfluorescein N-succinimidyl ester:

Fluorescence Microscopy: Widely employed in visualizing proteins, peptides, and other biomolecules, 6-FAM SE serves as a beacon under fluorescence microscopy. Its luminous characteristics enable researchers to trace and scrutinize the whereabouts and interactions of labeled molecules within cells. This pivotal application is indispensable for exploring cellular structures, unraveling molecular dynamics, and dissecting biological processes in real-time.

Flow Cytometry: At the forefront of cell analysis, 6-FAM SE plays a pivotal role in characterizing and sorting cells based on their fluorescence intensity. This dye facilitates the concurrent assessment of various parameters, including cell size, granularity, and the presence of specific biomarkers. Flow cytometry is an invaluable tool for immunophenotyping, cell sorting, and studying diverse cell populations with intricate compositions.

Quantitative PCR (qPCR): Serving as a beacon in quantitative PCR assays, 6-FAM SE acts as a fluorescent reporter dye embedded in probes. By integrating 6-FAM SE into the probes, researchers can monitor the real-time amplification of target DNA sequences through fluorescence detection. This sophisticated technique enables precise quantification of nucleic acids, aiding in gene expression analyses, pathogen identification, and genetic variability assessments.

Protein-Protein Interaction Studies: In the realm of deciphering biological interactions, 6-FAM SE labeling emerges as a potent tool for probing protein-protein interactions. By tagging a protein with 6-FAM SE, researchers can track its engagements with other biomolecules utilizing techniques like fluorescence resonance energy transfer (FRET). This method provides invaluable insights into the intricate dynamics and mechanisms governing biological interactions, shedding light on the interplay within complex biological systems.