Oxonol V | 61389-30-8

Oxonol V, a membrane potential-sensitive dye, finds widespread application in scientific research. Here are four key uses of Oxonol V:

Cell Viability Assays: In cell culture experiments, Oxonol V plays a pivotal role in assessing cell viability and membrane potential alterations. By introducing Oxonol V, researchers can precisely determine the proportion of viable cells through fluorescence intensity analysis. This tool proves invaluable in investigations concerning drug toxicity and the assessment of cytoprotective agents.

Mitochondrial Function Studies: Delving into live cells, Oxonol V offers a pathway to explore mitochondrial membrane potential, especially in cells exhibiting depolarized mitochondria. With its unique property of accumulation in dysfunctional mitochondria, this dye aids in identifying mitochondrial irregularities. Such insights are crucial for understanding diseases associated with mitochondrial dysfunction, such as neurodegenerative conditions and metabolic syndromes.

Electrophysiology Research: Within the realm of electrophysiology, Oxonol V emerges as a vital tool for visualizing and quantifying changes in membrane potential. Researchers leverage this dye to track the dynamics of ion channel activity and action potentials in excitable cells. This information proves essential for unraveling the electrical characteristics of tissues and paving the way for the development of innovative pharmaceuticals targeting ion channels.

Flow Cytometry: By utilizing Oxonol V in flow cytometry, researchers gain a powerful tool for assessing changes in membrane potential at a single-cell level. Staining cells with this dye enables the distinction of different cell populations based on their membrane potential status. This technique proves invaluable in diverse fields such as immunology, oncology, and cell biology research, aiding in the characterization of cell health and functional status.