1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate | 41085-99-8

1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate, more commonly known as DiI, serves as a prominent fluorescent dye in bioscience research. Here are four key applications of DiI:

Neurobiology: DiI emerges as a pivotal tool for labeling and tracing neurons, along with their intricate connections within the nervous system. By introducing DiI to neural tissues through injections or applications, researchers unlock the ability to visualize the intricate architecture and pathways of neurons under the discerning gaze of a fluorescence microscope. This application stands as a cornerstone for unraveling the mysteries of neural development, connectivity, and the impacts of various neurological disorders.

Membrane Studies: Delving into the realm of cell membranes, DiI shines brightly due to its exceptional affinity for lipid bilayers. Researchers harness its potential to label and monitor membrane dynamics, fusion events, and cell-cell interactions with unparalleled precision. This unique property proves invaluable in scrutinizing complex processes like endocytosis, exocytosis, and cell migration, shedding light on the intricacies of cellular behavior.

Histology: Within the realm of histology, DiI takes center stage by offering an illuminating perspective on fixed tissue samples through detailed fluorescence labeling of cell membranes. This enhancement significantly boosts the contrast and clarity of tissue structures, facilitating a deeper investigation into cellular organization and pathology. Complementing other fluorescent markers, DiI staining enables researchers to engage in multi-color imaging, unlocking a spectrum of detailed insights.

Cell Tracking: Facilitating cell tracking endeavors, DiI plays a pivotal role in monitoring the dynamic movements and proliferation of cells across various temporal scales. By integrating DiI into the membranes of live cells, scientists gain the ability to trail these cellular entities within in vitro or in vivo settings, leveraging fluorescence imaging techniques for precise observation. This application serves as a critical linchpin for studies spanning diverse domains like wound healing, immune responses, and the metastatic cascade of cancer cells.