Bis-(1,3-diethylthiobarbituric acid)trimethineoxonol | 47623-98-3

Bis-(1,3-diethylthiobarbituric acid) trimethineoxonol, commonly known as DiSBAC2(3), is a membrane potential-sensitive dye with diverse applications in bioscience. Here are four key applications of Bis-(1,3-diethylthiobarbituric acid) trimethineoxonol:

Membrane Potential Studies: DiSBAC2(3) serves as a versatile tool for investigating changes in membrane potential across various cell types. By integrating into the lipid bilayer, the dye undergoes fluorescence alterations corresponding to membrane depolarization and hyperpolarization. This unique property enables researchers to track the electrical dynamics of cells in real-time, offering invaluable insights into neuronal activity and cardiac physiology.

Drug Screening: Employed in high-throughput screening assays, DiSBAC2(3) aids in the evaluation of compound effects on cellular excitability. Through the detection of shifts in membrane potential, researchers can pinpoint potential drug candidates that modulate ion channels or other membrane-associated proteins. This particular application holds significant promise in the realm of pharmaceutical development for neurological and cardiovascular conditions.

Toxicology Studies: DiSBAC2(3) emerges as a critical asset in assessing the impact of environmental toxins and pharmaceutical agents on cellular well-being. Changes in membrane potential serve as key indicators of cell viability and functionality under toxic stressors. This makes the dye a pivotal instrument in exploring cellular responses to harmful substances and ensuring the safety profile of novel chemical entities.

Microbial Physiology: Within the field of microbiology, DiSBAC2(3) finds utility in probing membrane potential dynamics within bacteria and other microorganisms. Through the application of this dye, researchers can investigate how various environmental factors or antimicrobial agents influence bacterial membrane potential. This knowledge is fundamental for deciphering microbial behaviors, understanding pathogenicity, and crafting innovative antimicrobial strategies to combat infectious diseases.