FAM Maleimide

Product Introduction

FAM Maleimide is a thiol reactive bright green dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 494/520 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. Maleimides selectively label thiols of cysteine residue via 1,4-addition pathway, without interacting with amines, to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they might react with primary amines under strong basic environment. We offer FAM maleimide for labeling of thiols on antibodies, peptides, proteins and nucleotide.

Chemical Information
Synonyms	5-FAM Maleimide
IUPAC Name	N-[2-(2,5-dioxopyrrol-1-yl)ethyl]-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxamide
Canonical SMILES	C1=CC2=C(C=C1C(=O)NCCN3C(=O)C=CC3=O)C(=O)OC24C5=C(C=C(C=C5)O)OC6=C4C=CC(=C6)O
InChI	InChI=1S/C27H18N2O8/c30-15-2-5-19-21(12-15)36-22-13-16(31)3-6-20(22)27(19)18-4-1-14(11-17(18)26(35)37-27)25(34)28-9-10-29-23(32)7-8-24(29)33/h1-8,11-13,30-31H,9-10H2,(H,28,34)
InChI Key	INANBQGJVQTBLY-UHFFFAOYSA-N
Solubility	DMF, DMSO
Appearance	Yellow solid

Computed Properties
XLogP3	2
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	4
Exact Mass	498.10631554 g/mol
Monoisotopic Mass	498.10631554 g/mol
Topological Polar Surface Area	143Ų
Heavy Atom Count	37
Formal Charge	0
Complexity	968
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	1
Compound Is Canonicalized	Yes